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Novel Method for HPLC Analysis of Triterpenic Acids Using 9-Anthryldiazomethane Derivatization and Fluorescence Detection

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Abstract

Triterpenic acids are a group of secondary plant metabolites which are part of the cuticular waxes covering fruits, leaves, and flowers. To date, quantitative analysis of these compounds has often been conducted using high-performance liquid chromatography coupled with spectrophotometric detection or mass spectrometry; however, these methods have some major drawbacks. This paper reports a new method of analysis implementing derivatization with 9-anthryldiazomethane and fluorescence detection. The method consists of the extraction of analytes from a matrix, purification with anion exchanging SPE columns, and an optional step of the alkaline hydrolysis of triterpenic acid esters. The paper also describes a fast and easy method for the synthesis of the derivatization agent. The detection limits of the method presented are approximately 100-fold lower than in a similar method using ultraviolet spectrophotometry as the mode of detection. The recovery and repeatability of the method are at satisfactory levels.

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Authors and Affiliations

  1. Department of Fruit and Vegetable Product Technology, Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, 36 Rakowiecka St., 02-532, Warsaw, Poland

    Łukasz Woźniak, Krystian Marszałek & Sylwia Skąpska

  2. Department of Food Analysis, Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, 36 Rakowiecka St., 02-532, Warsaw, Poland

    Renata Jędrzejczak

Authors
  1. Łukasz Woźniak
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  2. Krystian Marszałek
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  3. Sylwia Skąpska
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  4. Renata Jędrzejczak
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Correspondence to Łukasz Woźniak.

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Woźniak, Ł., Marszałek, K., Skąpska, S. et al. Novel Method for HPLC Analysis of Triterpenic Acids Using 9-Anthryldiazomethane Derivatization and Fluorescence Detection. Chromatographia 80, 1527–1533 (2017). https://doi.org/10.1007/s10337-017-3371-6

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  • DOI: https://doi.org/10.1007/s10337-017-3371-6

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