Abstract
In this study, a new capillary electrophoresis (CE) method was developed for the online determination of platelet aggregation induced by thrombin (THR). The developed CE method was applied to evaluate the anti-platelet aggregation activity of Rhizoma Corydalis extract. Besides, the anti-platelet activity of Rhizoma Corydalis extract was also evaluated by the classic turbidimetry method for comparison. The results showed that platelet aggregation rate could be obtained or calculated based on the peak height or peak area of free platelets in CE analysis, and the anti-platelet aggregation activities of Rhizoma Corydalis extract were both dose-dependent manner determined by the CE and turbidimetry methods. Furthermore, compared with classic turbidimetry for platelet aggregation assay, CE is superior in smaller sample consumption and higher repeatability. Therefore, CE may be a potential method for evaluating anti-platelet aggregation activity of drugs.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (21275169 and 81202886), the Natural Science Foundation Project of CQ CSTC (cstc2015jcyjA10044), project No. CQDXWL-2014-Z007 supported by the Fundamental Research Funds for the Central Universities. We gratefully acknowledge the University of Macau for financial support for this research by the project MYRG2016-00144-ICMS-QRCM.
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All animal experimental procedures were approved by the Institutional Animal Ethical Committee of Chongqing University and were conducted according to the Guide for the Care and Use of Laboratory Animal of the National Institute of Health (Publication No. 80-23, revised 1996).
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Wang, FQ., Li, QQ., Zhang, Q. et al. Determination of Platelet Aggregation by Capillary Electrophoresis. Chromatographia 80, 341–345 (2017). https://doi.org/10.1007/s10337-016-3231-9
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DOI: https://doi.org/10.1007/s10337-016-3231-9