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Ionic-Liquid-Based Infrared-Assisted Extraction (IL-IRAE) Coupled with HPLC–MS: a Green and Convenient Tool for Determination of TCMs

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Abstract

Infrared radiation, one form of electromagnetic wave, has potential value due to its heating effect, but has become a matter of grave concern in recent years. We describe herein ionic-liquid-based infrared-assisted extraction (IL-IRAE) coupled with high-performance liquid chromatography (HPLC)–mass spectrometry (MS) as a green and convenient method for simultaneous determination of three alkaloids in Lycoris radiata, a traditional Chinese medicine (TCM). Extraction parameters including the type and concentration of IL, extraction solvent, liquid/solid ratio, extraction time, and irradiation power were optimized via orthogonal design and analysis of variance (ANOVA). Chromatography–mass spectrometry was performed in positive ion mode on a TSK gel C18 column (150 mm × 4.6 mm id, 5-µm particle size) with less than 5-min separation. Evaluation of the method showed good results with analytical recovery ranging from 100.66 to 103.90 %. The extraction efficiency of IL-IRAE was proved to be increased by 25 % compared with conventional heat reflux extraction (HRE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) methods under the operational parameters investigated in the study, indicating that the developed IL-IRAE method is environmentally friendly, simple, low cost, and efficient, offering great promise for quick determination of active compounds in TCMs and natural plants.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81473175).

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    1. Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, No. 826 Zhangheng Road, Shanghai, 201203, People’s Republic of China

      Chen Xu, Li Ling, Jiajun Zhu, Jiakun Long, Gengli Duan & Yingjia Yu

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    1. Chen Xu
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    2. Li Ling
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    3. Jiajun Zhu
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    4. Jiakun Long
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    5. Gengli Duan
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    6. Yingjia Yu
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    Correspondence to Gengli Duan or Yingjia Yu.

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    This article does not contain any studies with human participants or animals performed by any of the authors.

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    C. Xu and L. Ling contributed equally to this work.

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    Xu, C., Ling, L., Zhu, J. et al. Ionic-Liquid-Based Infrared-Assisted Extraction (IL-IRAE) Coupled with HPLC–MS: a Green and Convenient Tool for Determination of TCMs. Chromatographia 80, 335–340 (2017). https://doi.org/10.1007/s10337-016-3230-x

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