Chromatographia

, Volume 80, Issue 5, pp 705–730 | Cite as

Necessary Analytical Skills and Knowledge for Identifying, Understanding, and Performing HPLC Troubleshooting

Review
Part of the following topical collections:
  1. Young Investigators in Separation Science

Abstract

This review paper summarizes the main topics discussed by John Dolan in the LC troubleshooting series published over the last 16 years in LCGC North America. The paper is directed toward non-expert HPLC users to learn from challenges other HPLC users across the world faced. The topics covered here help the reader not only to understand and perform system troubleshooting but also to learn tips and essentials for data analysis, method development, and system testing (for example: proper integration, establishing best calibration curve, enhancing signal-to-noise ratio, improving selectivity, testing pump performance, etc.). The last section of the paper shows helpful tips, shortcuts, and equations that would be very helpful to remember, for example: (1) simplified equations for calculating dead volume and column efficiency, (2) easy-to-remember rules on how to set detector constant and maximum injection volume, (3) helpful tips for method development, such as choosing gradient or isocratic conditions, as well as (4) recommendations on how to take care of the HPLC system and columns. This review paper can also be useful guide for HPLC users to find references of particular interest from the LC troubleshooting series.

Keywords

LC troubleshooting Data analysis John Dolan LC troubleshooting Method development LC tips and tricks HPLC system tests 

Notes

Acknowledgements

The author would like to acknowledge Dr. Arianne Soliven, Dr. Adrian Clarke, Frank Hrovat, and James Tam for their helpful suggestions.

Compliance with Ethical Standards

No funding for this work was provided.

Conflict of interest

The author declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by the authors.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Novartis Pharmaceuticals CorporationSan CarlosUSA

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