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Necessary Analytical Skills and Knowledge for Identifying, Understanding, and Performing HPLC Troubleshooting

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Abstract

This review paper summarizes the main topics discussed by John Dolan in the LC troubleshooting series published over the last 16 years in LCGC North America. The paper is directed toward non-expert HPLC users to learn from challenges other HPLC users across the world faced. The topics covered here help the reader not only to understand and perform system troubleshooting but also to learn tips and essentials for data analysis, method development, and system testing (for example: proper integration, establishing best calibration curve, enhancing signal-to-noise ratio, improving selectivity, testing pump performance, etc.). The last section of the paper shows helpful tips, shortcuts, and equations that would be very helpful to remember, for example: (1) simplified equations for calculating dead volume and column efficiency, (2) easy-to-remember rules on how to set detector constant and maximum injection volume, (3) helpful tips for method development, such as choosing gradient or isocratic conditions, as well as (4) recommendations on how to take care of the HPLC system and columns. This review paper can also be useful guide for HPLC users to find references of particular interest from the LC troubleshooting series.

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Acknowledgements

The author would like to acknowledge Dr. Arianne Soliven, Dr. Adrian Clarke, Frank Hrovat, and James Tam for their helpful suggestions.

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Authors and Affiliations

  1. Novartis Pharmaceuticals Corporation, San Carlos, USA

    Imad A. Haidar Ahmad

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  1. Imad A. Haidar Ahmad
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Correspondence to Imad A. Haidar Ahmad.

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Published in the topical collection Young Investigators in Separation Science with editors D. Mangelings, G. Massolini, G. K. E. Scriba, R. M. Smith, and A. M. Striegel.

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Haidar Ahmad, I.A. Necessary Analytical Skills and Knowledge for Identifying, Understanding, and Performing HPLC Troubleshooting. Chromatographia 80, 705–730 (2017). https://doi.org/10.1007/s10337-016-3225-7

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