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Resolving Co-Elution Problems of Components in Complex Mixtures by Multiple Heart-Cutting 2D-LC

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Abstract

Multiple heart-cutting (MHC) 2D-LC was applied to resolve challenging peak co-elutions in a pesticidal natural product-derived extract, which exhibited a complex impurity profile. While the 1D separation using sub-2 μm particle column technology provided good separation efficiency, additional selectivity was required to separate overlapping peaks in several chromatographic regions. MHC 2D-LC using C18 columns in both dimensions, but different pH in the mobile phase, provided full separation of the components of interest. This article describes how the MHC 2D-LC technology has been applied for optimized analysis of target components as well as being a rapid tool for peak purity evaluation. It also discusses strategic benefits of the technology regarding method development for separation of very complex samples.

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Acknowledgements

This work was in part funded by the Technology Development and Renewal (TRD) program in the Dow Analytical Technology Center.

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Authors and Affiliations

  1. Dow Deutschland Anlagen GmbH, Analytical Sciences, 21677, Stade, Germany

    Matthias Pursch

  2. Dow AgroSciences LLC, Bioengineering and Bioprocessing R&D, Indianapolis, IN, 46268, USA

    Paul Lewer

  3. Agilent Technologies, Research and Development, Hewlett-Packard-Str. 8, 76337, Waldbronn, Germany

    Stephan Buckenmaier

Authors
  1. Matthias Pursch
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  2. Paul Lewer
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  3. Stephan Buckenmaier
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Correspondence to Matthias Pursch.

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All authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Dedicated to Prof. Dr. Klaus Albert on the occasion of his 70th birthday.

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Pursch, M., Lewer, P. & Buckenmaier, S. Resolving Co-Elution Problems of Components in Complex Mixtures by Multiple Heart-Cutting 2D-LC. Chromatographia 80, 31–38 (2017). https://doi.org/10.1007/s10337-016-3214-x

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  • DOI: https://doi.org/10.1007/s10337-016-3214-x

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