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Multi-Residue Method for the Screening of Benzimidazole and Metabolite Residues in the Muscle and Liver of Sheep and Cattle Using HPLC/PDAD with DVB-NVP-SO3Na for Sample Treatment

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Abstract

A high-performance liquid chromatography (HPLC) screening method with a photodiode array detector (PDAD) was established for the simultaneous determination of residues of 13 benzimidazoles (BZDs) and metabolites in sheep and cattle muscle and liver. Samples were extracted by ultrasonication in ethyl acetate and purified over DVB-NVP-SO3Na sorbent. Under the optimized conditions, good linearities were obtained for BZDs and metabolites with correlation coefficients (R 2) greater than 0.9911. The recoveries of the 13 BZDs and metabolites from spiked samples were 72.0–119.3 %, with intraday and interday relative standard deviations (RSDs) below 22.8 %. The limits of detection (LODs) and quantitation (LOQs) were 0.8–4.9 and 2.6–18.2 μg kg−1, respectively. The results clearly demonstrated that the developed approach enables reliable screening of 12 BZDs and metabolites except flubendazole (FLU) and could be used as a regulatory tool for the screening of BZD and metabolite residues in the muscle and liver of sheep and cattle.

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Acknowledgments

This study was funded by research Grants from the Chinese Academy of Agricultural Sciences (No. 1610322014014).

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Authors and Affiliations

  1. Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, 335 Jiangouyan St, Lanzhou, Gansu, China

    Lin Xiong, Shimo Peter Shimo, Weihong Li, Xiaolin Yang & Ping Yan

  2. Laboratory of Quality and Safety Risk Assessment for Livestock Product (Lanzhou), Ministry of Agriculture, 335 Jiangouyan St, Lanzhou, Gansu, China

    Lin Xiong, Shimo Peter Shimo, Weihong Li, Xiaolin Yang & Ping Yan

  3. Department of Unclear Medicine, Lanzhou University Second Hospital, 82 Cuiying St, Lanzhou, Gansu, China

    Lele Huang

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  1. Lin Xiong
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  2. Lele Huang
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  4. Weihong Li
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  5. Xiaolin Yang
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Correspondence to Lin Xiong.

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The authors have no conflicts of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Xiong, L., Huang, L., Shimo, S.P. et al. Multi-Residue Method for the Screening of Benzimidazole and Metabolite Residues in the Muscle and Liver of Sheep and Cattle Using HPLC/PDAD with DVB-NVP-SO3Na for Sample Treatment. Chromatographia 79, 1373–1380 (2016). https://doi.org/10.1007/s10337-016-3144-7

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  • DOI: https://doi.org/10.1007/s10337-016-3144-7

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