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Determination of 19 Representative Pesticides in Traditional Chinese Medicines by Dispersive Liquid-Liquid Microextraction and Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

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Abstract

The objective of this study was to develop an analytical method based on ultrasonic-assisted dispersive liquid–liquid microextraction (DLLME) for the determination of 19 pesticides in Shengmaiyin and its resulting crude drugs Ophiopogon japonicas and Codonopis pilosula. The target compounds were qualitatively and quantitatively studied via ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) operated in multiple reaction-monitoring modes. The UHPLC–MS/MS conditions were optimized to complete chromatographic separation in 9 min. The correlation coefficient was higher than 0.993 for each pesticide. Average recoveries of the 19 compounds from different fortified samples ranged between 81 and 108 % with inter-RSD values below 13 %. The limits of quantification (LOQs) for 19 compounds were in the range of 0.0075–0.81 μg kg−1. Recovery LOQs of the DLLME method were compared with the routine QuEChERS method, and the results showed that the proposed method is very simple, rapid, and sensitive. This makes it a more applicable method for monitoring multiple classes of pesticides in traditional Chinese medicine.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31170326, 31370374), team project of Jilin Provincial Science, Technology Department (No. 20130413043 GH) and Natural Science Foundation of Changchun Normal University.

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Authors and Affiliations

  1. Central Laboratory, Changchun Normal University, Changchun, 130032, People′s Republic of China

    Yao Qin, Lina Chen, Xiaojing Yang, Ying Tang, Senlin Li & Chunming Liu

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  1. Yao Qin
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  2. Lina Chen
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  3. Xiaojing Yang
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  4. Ying Tang
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  5. Senlin Li
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  6. Chunming Liu
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Correspondence to Chunming Liu.

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Qin, Y., Chen, L., Yang, X. et al. Determination of 19 Representative Pesticides in Traditional Chinese Medicines by Dispersive Liquid-Liquid Microextraction and Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry. Chromatographia 79, 875–884 (2016). https://doi.org/10.1007/s10337-016-3105-1

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