Chromatographia

, Volume 79, Issue 17–18, pp 1061–1068 | Cite as

Metabolite Profiling to Monitor Organochlorine Pesticide Exposure in HepG2 Cell Culture

  • Martha Zuluaga
  • Jhon Jairo Melchor
  • Fredy Alexander Tabares-Villa
  • Gonzalo Taborda
  • Juan Carlos Sepúlveda-Arias
Original
Part of the following topical collections:
  1. 5th Latin American Pesticide Residue Workshop

Abstract

Gas chromatography coupled with time of flight mass spectrometry (GC/MS-TOF) was used to profile endogenous metabolites in HepG2 cell cultures to assess the metabolic changes induced by exposure to different organochlorine pesticides, their mixtures and controls (endosulfan, lindane, DDT and aldrin). Cells were cultured in DMEM with Glutamax at 37 °C with 5 % CO2 for 72 h and then exposed to each pesticide, pesticide mixture or DMSO (as a control) for 24 h, and finally, endogenous metabolites were extracted and analyzed using GC/MS-TOF. The experiment was repeated six times under the same cell passage and culture conditions. PCA, PLS-DA and ROC were performed to analyze the GC/MS-TOF data and identify potential biomarkers. Thirty-five explanatory metabolites were found in both PCA and PLS-DA models, where Q 2 was 0.86 and R 2 was 0.98. Univariate and multivariate ROC showed potential biomarkers for each treatment, suggesting a general toxic mechanism for organochlorine pesticides that is specific for each type of compound. These results confirmed the effect of OCPs in sugar and amino acid metabolism that are linked with the function of cytochrome P450 in reductive dechlorination and oxidative stress.

Keywords

Organochlorine Pesticides Metabolite profiling Biomarkers Metabolomics 

Notes

Acknowledgments

To Becas Generación de Bicentenario conv. 511 from Colciencias (Grant to Martha Zuluaga). To Programa de Jóvenes Investigadores e Innovadores from Colciencias and Universidad Tecnológica de Pereira (Grant to Melchor JJ and Tabares-Villa FA, contracts 5348/2013 and 5826/2013, respectively) and the West Coast Metabolomics Center for receiving Martha Zuluaga as a visitor scholar during 2014.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10337_2016_3031_MOESM1_ESM.pdf (714 kb)
Supplementary Figure 1 ROC of each treatment Vs control and PLS-DA features. a Endosulfan Vs Control ROC. b Endosulfan Biomarkers. c Aldrin Vs Control ROC d Aldrin Biomarkers e DDT Vs control ROC. f DDT Biomarkers g Lindane Vs Control ROC h Lindane Biomarkers i Mixture of pesticides Vs Control ROC j Mixture of pesticides Biomarkers (PDF 713 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Martha Zuluaga
    • 1
  • Jhon Jairo Melchor
    • 2
  • Fredy Alexander Tabares-Villa
    • 2
  • Gonzalo Taborda
    • 1
  • Juan Carlos Sepúlveda-Arias
    • 2
  1. 1.Grupo de Investigación en Cromatografía y Técnicas Afines, Departamento de QuímicaFacultad de Ciencias Exactas y Naturales, Universidad de CaldasManizalesColombia
  2. 2.Grupo Infección e Inmunidad, Departamento de Ciencias BásicasFacultad de Ciencias de la Salud, Universidad Tecnológica de PereiraPereiraColombia

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