Abstract
A simple microextraction, namely low-density solvent with low-toxicity organic solvent-based dispersive liquid–liquid microextraction (LDS-DLLME) and modified QuEChERS sample preparation procedures were optimized and validated for preconcentration of neonicotinoid pesticides prior to the determination using HPLC. The experimental parameters affecting the extraction efficiency, including salt addition, type of disperser solvent and its volume, effect of extraction solvent and its volume, and extraction time were investigated. Under the selected LDS-DLLME and HPLC conditions, separation of seven neonicotinoid pesticides (imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, dinotefuran, and nitenpyram) was achieved within 26 min. 1-Octanol (extraction solvent) and acetonitrile (disperser solvent) were used for extraction of the target analytes. Under the optimum condition, linearity was obtained within the range of 0.1–1000 ng g−1 with a correlation coefficient more than 0.999. The high enrichment factor of the target analytes was 50-fold and a low limit of quantitation (0.80–2.50 ng g−1) could be obtained. The fruit samples (at fortified levels of 10, 30, and 50 ng g−1) were successfully analyzed, and relative recoveries were obtained in the range of 90.07–112.22 %.
Similar content being viewed by others
References
Sánchez-Bayo F, Hyne RV (2014) Chemosphere 99:143–151
Zhang S, Yang X, Yin X, Wang C, Wang Z (2012) Food Chem 133:544–550
Di Muccio A, Fidente P, Barbini DA, Dommarco R, Seccia S, Morrica P (2006) J Chromatogr A 1108:1–6
Pesticide EUMRLs Database (2008)
Wu Q, Li Z, Wang C, Wu C, Wang W, Wang Z (2011) Food Anal Methods 4:559–566
Rancan M, Sabatini AG, Achilli G, Galletti GC (2006) Anal Chim Acta 555:20–24
Gil MD, Martínez M, Santiago R, Galanti A, Girotti S (2007) J Chromatogr A 1147:17–23
Pareja L, Colazzo M, Pérez-Parada A, Niell S, Carrasco-Letelier L, Besil N, Cesio MV, Heinzen H (2011) Int J Environ Res Public Health 8:3844–3858
Tapparo A, Giorio C, Soldà L, Bogialli S, Marton D, Marzaro M, Girolami V (2013) Anal Bioanal Chem 405:1007–1014
Jovanov P, Guzsvány V, Franko M, Lazić S, Sakač M, Milovanović I, Nedeljković N (2014) Food Res Int 55:11–19
Vichapong J, Burakham R, Srijaranai S (2013) Talanta 117:221–228
Jansson C, Pihlstrom T, Öterdahl BG, Markides KE (2004) J Chromatogr A 1023:93–104
Zhou Q, Ding Y, Xiao J (2006) Anal Bioanal Chem 385:1520–1525
Štajnbaher D, Zupančič-Kralj L (2003) J Chromatogr A 1015:185–198
Cazorla-Reyes R, Fernandez-Moreno JL, Romero-Gonzalez R, Frenich AG, Vidal JL (2011) Talanta 85:183–196
Liu S, Zheng Z, Wei F, Ren Y, Gui W, Wu H, Zhu G (2010) J Agric Food Chem 58:3271–3278
Chitescu CL, Oosterink E, de Jong J, Stolker AAML (2012) Talanta 88:653–662
Yáñez KP, Bernal JL, Nozal MJ, Martín MT, Bernal J (2013) J Chromatogr A 1285:110–117
Campillo N, Viñas P, Férez-Melgarejo G, Hernández-Córdoba M (2013) J Agric Food Chem 61:4799–4805
Jovanov P, Guzsvány V, Franko M, Lazić S, Sakač M, Šarić B, Banjac V (2013) Talanta 111:125–133
Leong M-I, Huang S-D (2008) J Chromatogr A 1211:8–12
Vera-Avila LE, Rojo-Portillo T, Covarrubias-Herrera R, Peña-Alvarez A (2013) Anal Chim Acta 805:60–69
Ahmadi-Jouibari T, Fattahi N, Shamsipur M (2014) J Pharm Biomed Anal 94:145–151
Sanagi MM, Abbas HH, Ibrahim WAW, Aboul-Enien HY (2012) Food Chem 133:557–562
Xu H, Ding Z, Lv L, Song D, Feng Y-Q (2009) Anal Chim Acta 636:28–33
Nguyen TD, Yu JE, Lee DM, Lee G-H (2008) Food Chem 110:207–213
Banerjee K, Oulkar DP, Dasgupta S, Patil SB, Patil SH, Savant R, Adsule PG (2007) J Chromatogr A 1173:98–109
Seebunrueng K, Santaladchaiyakit Y, Srijaranai S (2012) Anal Bioanal Chem 404:1539–1548
Acknowledgments
This article is dedicated to Professor Dr. Kate Grudpan (Chiang Mai University, Thailand) in celebration of his 60th birthday. The authors gratefully acknowledge the financial supports of this research by the Thailand Research Fund (TRF) and Mahasarakham University, through the TRF Grant for New Scholars (Grant no. TRG5780060). The authors also gratefully acknowledge the partial supports for this research from the Center for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, and Materials Chemistry Research Center, Khon Kaen University.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Vichapong, J., Burakham, R. & Srijaranai, S. Alternative Liquid–Liquid Microextraction as Cleanup for Determination of Neonicotinoid Pesticides Prior HPLC Analysis. Chromatographia 79, 285–291 (2016). https://doi.org/10.1007/s10337-016-3022-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-016-3022-3