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Potential of Online Comprehensive Two-Dimensional Liquid Chromatography For Micro-Preparative Separations of Simple Samples

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Abstract

Generally online comprehensive two-dimensional liquid chromatography (LC × LC) is used at preparative scale to prepare pure compounds from very complex samples which cannot be separated by one-dimensional liquid chromatography (1D-LC), even at analytical scale. In this work, micro-preparative online LC × LC was evaluated for the recovery of minor components from a simple mixture which can be separated by 1D-LC. This study is a proof of concept made from a test sample of four compounds which were separated by two 1D-LC methods and an LC × LC method based on the previous 1D-LC ones. The comparison of the preparative performance of the three methods shows that online LC × LC was able to achieve a 12-fold gain in amount recovered per injection compared to 1D-LC.

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Authors and Affiliations

  1. Institut des Sciences Analytiques, Université de Lyon, UMR CNRS UCBL ENS 5280, 5 rue de La Doua, 69100, Villeurbanne, France

    Amélie Corgier, Morgan Sarrut, Gérard Crétier & Sabine Heinisch

Authors
  1. Amélie Corgier
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  2. Morgan Sarrut
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  3. Gérard Crétier
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  4. Sabine Heinisch
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Correspondence to Sabine Heinisch.

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Corgier, A., Sarrut, M., Crétier, G. et al. Potential of Online Comprehensive Two-Dimensional Liquid Chromatography For Micro-Preparative Separations of Simple Samples. Chromatographia 79, 255–260 (2016). https://doi.org/10.1007/s10337-015-3012-x

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  • DOI: https://doi.org/10.1007/s10337-015-3012-x

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