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Quantitative Analysis of Melissa officinalis L. Samples by Chromatographic Multivariate Calibration Methods

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Abstract

In this study, improved chromatographic multivariate calibration methods were developed for the quantitative analysis of caffeic acid (CAA) and rosmarinic acid (RA) in 12 different plant samples of Melissa officinalis L. (lemon balm), a perennial herb which belongs to the Lamiaceae family and is a well-known medicinal plant. UPLC (ultra performance liquid chromatography) separation was performed on a Waters UPLC BEH phenyl column (100 mm × 2.1 mm i.d., 1.7 μm) using a mobile phase consisting of acetonitrile, 0.1 M formic acid buffer (pH 3.80), and acetic acid buffer (pH 4.74) (17:76:7, v/v) in the presence of 1.0 mL triethylamine in 1000 mL mobile phase. Multiwavelength chromatograms were obtained by photodiode array (PDA) detection in the wavelength range 275–345 nm at intervals of Δλ = 10 nm. Partial least squares (PLS) and principal component regression (PCR) analyses were utilized by applying PCR and PLS algorithms to the concentration set and to the multiwavelength peak areas for both CAA and RA. The proposed UPLC-PCR and UPLC-PLS methods permitted the accurate quantification of CAA and RA in 12 samples of M. officinalis obtained from different herbalists in several Turkish cities.

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Acknowledgments

Analytical experiments and processes were performed at the Chemometric Laboratory of the Faculty of Pharmacy, which was supported by the scientific research fund of Ankara University (Project Number 10A3336001). The authors would like to thank Ankara University for their support during the completion of this study.

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara University, Tandoğan, 06100, Ankara, Turkey

    Gülsen Kendir & Ayşegül Köroğlu

  2. Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Tandoğan, 06100, Ankara, Turkey

    Erdal Dinç

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  1. Gülsen Kendir
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  2. Erdal Dinç
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Correspondence to Erdal Dinç.

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Kendir, G., Dinç, E. & Köroğlu, A. Quantitative Analysis of Melissa officinalis L. Samples by Chromatographic Multivariate Calibration Methods. Chromatographia 79, 189–198 (2016). https://doi.org/10.1007/s10337-015-3005-9

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