, Volume 78, Issue 21–22, pp 1409–1413 | Cite as

Analysis of Glycosaminoglycan Oligosaccharides by Combined HPTLC/MALDI-TOF MS: Reduced Silica Gel Thickness Leads to Improved Spectral Qualities and Reduced Side Reactions

  • Katharina Lemmnitzer
  • Hans Griesinger
  • Rosmarie Süß
  • Katerina Matheis
  • Michael Schulz
  • Jürgen SchillerEmail author
Short Communication


Thin-layer chromatography (TLC) is a simple, fast and inexpensive separation method which can be applied to virtually all natural products including oligosaccharides. Unfortunately, however, the unequivocal identification of a TLC spot is normally difficult. Fortunately, this problem can be minimized when mass spectrometry (MS) such as matrix-assisted laser desorption and ionization time-of-flight is used to identify the TLC spots. This work is dedicated to the TLC/MS analysis of oligosaccharides derived from native chondroitin sulfate and hyaluronan. We will show that the thickness of the silica gel layer (200 versus 100 µm) has a tremendous influence on the quality of the mass spectra: a reduced silica gel thickness enhances the spectral quality and, in particular, improves the achievable signal-to-noise ratio. Additionally, unwanted formylation of the GAG oligosaccharides (which occurs due to the high moiety of formic acid in the mobile phase) can also be minimized if MS-grade HPTLC plates are used.


Oligosaccharides Hyaluronan Chondroitin sulfate MALDI-TOF MS HPTLC Formylation 



This work was supported by the German Research Council (DFG Schi 476/16-1 as well as TR 67, project A2 and A8). We would like to thank Dr. Detlev Suckau and Dr. Martin Schürenberg (Bruker Daltonics, Bremen, Germany) for many helpful discussions.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Katharina Lemmnitzer
    • 1
  • Hans Griesinger
    • 2
  • Rosmarie Süß
    • 1
  • Katerina Matheis
    • 2
  • Michael Schulz
    • 2
  • Jürgen Schiller
    • 1
    Email author
  1. 1.Institute of Medical Physics and Biophysics, Medical FacultyUniversity of LeipzigLeipzigGermany
  2. 2.Merck KGaADarmstadtGermany

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