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Separation Study of Eight Isoflavones by MEKC with Different Surfactants

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Abstract

Isoflavones are a very important group of natural products. This study investigated the separation of eight isoflavones, namely ononin, daidzin, genistin, biochanin A, formononetin, puerarin, genistein, and daidzein, from pueraria by micellar electrokinetic chromatography (MEKC) with different surfactants. The following micellar systems of MEKC were systematically compared for the analysis of these isoflavones: (1) a single surfactant comprising the anionic surfactant sodium dodecyl sulfate (SDS), the cationic surfactant hexadecyltrimethylammonium bromide, the neutral surfactant polyoxyethylene sorbitan monolaurate (Tween 20), and the ionic liquid-type surfactant (also a cationic surfactant) 1-dodecyl-3-methylimidazolium tetrafluoroborate (C12MIMBF4); (2) different single surfactants with 1-butyl-3-methylimidazolium tetrafluoroborate (BMImBF4) as an additive (modifier); and (3) mixed micelles of SDS + Tween 20 and C12MIMBF4 + Tween 20. Both SDS with BMImBF4 as additive and mixed micelles of SDS + Tween 20 had the highest separation efficiency for the eight investigated compounds. Furthermore, the SDS with BMImBF4 as additive was more stable (good repeatability of retention time and peak shape of analytes) than mixed micelles of SDS + Tween 20, which may be the result of a stabilizing effect of BMImBF4. Therefore, the final analytical conditions were 15 mM SDS added with 50 mM BMImBF4 in 30 mM sodium tetraborate (STB, pH 9.5) as running buffer; applied voltage, 20 kV; injection, 50 mbar for 5 s; cartridge temperature, 25 °C; compounds were detected at 260 nm. The developed method was fully validated (limit of detection, limit of quantification, intraday precision, inter-day precision, and recovery) and successfully applied to determine the eight analytes in three Radix Puerariae samples. The present study indicated that SDS with ionic liquids as additive in MEKC was suitable for the analysis of isoflavones.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21275169, 81202886 and 21175159), and Project No. CQDXWL-2014-Z007 supported by the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China

    Wen Xiao, Cen Chen, Qian Zhang, Qi-Hui Zhang, Zhi-Ning Xia & Feng-Qing Yang

  2. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, People’s Republic of China

    Yuan-Jia Hu

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  1. Wen Xiao
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  2. Cen Chen
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  3. Qian Zhang
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  4. Qi-Hui Zhang
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Correspondence to Qi-Hui Zhang or Feng-Qing Yang.

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Xiao, W., Chen, C., Zhang, Q. et al. Separation Study of Eight Isoflavones by MEKC with Different Surfactants. Chromatographia 78, 1385–1393 (2015). https://doi.org/10.1007/s10337-015-2969-9

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  • DOI: https://doi.org/10.1007/s10337-015-2969-9

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