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Development of a Rapid and Low Cost Method for Measuring Plasma Protein Binding

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Abstract

In drug discovery and development, it is very important to investigate the plasma protein binding (PPB) of a drug to better understand its in vivo fate. In this study, a rapid and low-cost solid-phase extraction (SPE) method was developed for determining the PPB. With this method, the total protein recovery of a blank human plasma sample was 83.7 %. The unbound drug was easily adsorbed by an ODS C18 SPE column, and the recovery of three known drugs was more than 90 %. Their PPBs obtained by the SPE were identical to the value reported by conventional techniques. In addition, more than 90 % of 4-amino-2-trifluoromethyl-phenyl retinate (ATPR), which is a novel all-trans retinoic acid derivative (ATRA), was bound to human plasma protein as determined by SPE, and this value was comparable with that obtained by our previously described gel filtration-based method. Considering its versatility, speed of separation, and low cost, SPE is a rapid and economical method for measuring PPB.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (no. 81274100), Natural Science Foundation of Anhui Province of China (no. 1408085QH189), and Key Project for the Excellent Higher Education of Anhui Province of China (no. 2013SQRL019ZD). Financial support was also provided by the Young and Middle-aged Academic Backbone from Anhui Medical University.

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  1. School of Pharmacy, Anhui Medical University, 81 Meishan Road, 230032, Hefei, China

    Jihui Tang, Jue Song, Xiaoyan Liu, Hongmei Zang, Feihu Chen & Jun Li

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  1. Jihui Tang
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  2. Jue Song
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  3. Xiaoyan Liu
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  4. Hongmei Zang
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  5. Feihu Chen
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  6. Jun Li
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Correspondence to Jun Li.

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Tang, J., Song, J., Liu, X. et al. Development of a Rapid and Low Cost Method for Measuring Plasma Protein Binding. Chromatographia 78, 1169–1174 (2015). https://doi.org/10.1007/s10337-015-2929-4

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  • DOI: https://doi.org/10.1007/s10337-015-2929-4

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