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Advances in Capillary Electrophoresis-Based Enzyme Assays

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Abstract

Capillary electrophoresis (CE) has become a flexible and accurate, high-efficiency analytical separation technique in many areas requiring only minute amounts of sample and chemicals. Thus, CE has also been recognized as a suitable technique to study enzymatic reactions including the determination of Michaelis–Menten kinetic data or the identification and characterization of inhibitors. The most often applied CE-based enzyme assay modes can be divided into two categories: (1) pre-capillary assays where incubations are performed offline followed by CE analysis of substrate(s) and/or product(s) and (2) in-capillary assays in which the enzymatic reaction and analyte separation are performed in the same capillary. In case of the in-capillary assays, the enzyme may be immobilized or in solution. The latter is also referred to as electrophoretically mediated microanalysis (EMMA), while in the case of immobilized enzyme the term immobilized enzyme reactor (IMER) is used. The present review summarizes the literature on CE-based enzyme assays published between January 2010 and April 2015. Immobilized enzyme reactors as well as microfluidic devices applied to the study of enzymatic activity will also be briefly addressed.

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Acknowledgments

The financial support of F. Belal by the Alexander von Humboldt Foundation is gratefully acknowledged.

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmaceutical/Medicinal Chemistry, Friedrich Schiller University Jena, Philosophenweg 14, 07743, Jena, Germany

    Gerhard K. E. Scriba

  2. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, 35516, Egypt

    Fathalla Belal

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Correspondence to Gerhard K. E. Scriba.

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Scriba, G.K.E., Belal, F. Advances in Capillary Electrophoresis-Based Enzyme Assays. Chromatographia 78, 947–970 (2015). https://doi.org/10.1007/s10337-015-2912-0

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