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Optimization of a Solid-Phase Extraction Method for the Determination of 12 Aminoglycosides in Water Samples Using LC–ESI–MS/MS

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Abstract

A new and simple method has been developed for determination and quantification of 12 aminoglycosides in environmental water samples. The method is based on solid-phase extraction (SPE) followed by liquid chromatography coupled with tandem mass spectrometry (LC–ESI–MS/MS) for analysis. After optimization of LC–ESI–MS/MS conditions, two SPE cartridges, reversed-phase C18 with pentafluoropropionic acid (PFPA) and polymeric mixed mode cation exchange cartridges, have been tested to determine aminoglycoside residues in water. Parameters affecting the extraction efficiency for the two cartridges such as eluent type, percolation volume and percentage of ion pair PFPA were optimized. Extraction on C18 cartridges with PFPA reagent showed a better result in terms of recovery (65–115 %) and relative standard deviation (0.5–15 %) relative to strong cation exchange. This method was validated and their limits of quantification were in the range 5–50 ng L−1. The method was applied to evaluate the occurrence of these compounds in influent and effluent wastewater treatment plant and hospital water samples in Lebanon. Gentamycin was found in hospital wastewater samples at a concentration of 30 ng L−1.

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Authors and Affiliations

  1. Laboratory for Analysis of Organic Compound LACO, National Council for Scientific Research CNRS, Lebanese Atomic Energy Commission LAEC, 11-8281, Riad El Solh, Beirut, 1107 2260, Lebanon

    Samia Mokh, Khaled El Hawari & Mohamad Al Iskandarani

  2. Faculty of Public Health I, Lebanese University, Hadath, Lebanon

    Rania Nassar & Mohamad Al Iskandarani

  3. Laboratory of Physico and Toxico Chemistry, Bordeaux University 1, CNRS, EPOC-LPTC-UMR 5255, 351 Cours de la Libération, 33405, Talence, France

    Hélène Budzinski

Authors
  1. Samia Mokh
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  2. Khaled El Hawari
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  3. Rania Nassar
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  4. Hélène Budzinski
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  5. Mohamad Al Iskandarani
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Correspondence to Mohamad Al Iskandarani.

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Mokh, S., El Hawari, K., Nassar, R. et al. Optimization of a Solid-Phase Extraction Method for the Determination of 12 Aminoglycosides in Water Samples Using LC–ESI–MS/MS. Chromatographia 78, 631–640 (2015). https://doi.org/10.1007/s10337-015-2877-z

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  • DOI: https://doi.org/10.1007/s10337-015-2877-z

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