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Quantitative Analysis of Structural Isomers of Linear Pentaamines by GC–MS

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Abstract

Structural isomers of long linear polyamines are often highly difficult to differentially quantify due to their structural similarities. In this study, we focused on pentaamines, aminopropylcanavalmine [H2N(CH2)3NH(CH2)4NH(CH2)3NH(CH2)4NH2, (3-4-3-4)] and N 1-aminopropyl-N 8-aminobutylspermidine [H2N(CH2)3NH(CH2)3NH(CH2)4NH(CH2)4NH2, (3-3-4-4)], which have never been separated. We analyzed a mixture of the heptafluorobutyryl derivatives of these pentaamines by gas chromatography–mass spectrometry (GC–MS) and found that the polyamine content and intensity of the unique fragment ions detected in MS spectra exhibited a strong correlation. The calibration curves of appropriate combinations of the peak intensities versus pentaamine mixing ratios showed high linearity (r > 0.99) in the mixing ratio range of 0–2.33. These data indicated that the pentaamine content in the pentaamine mixed solutions could be determined using appropriate calibration curves. Therefore, this method can potentially be applied to measure the pentaamine content of biological samples.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, 350-0295, Japan

    Takemitsu Furuchi, Ayano Tazawa, Sachihiko Numajiri & Masaru Niitsu

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  1. Takemitsu Furuchi
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  2. Ayano Tazawa
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  3. Sachihiko Numajiri
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  4. Masaru Niitsu
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Correspondence to Takemitsu Furuchi.

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Furuchi, T., Tazawa, A., Numajiri, S. et al. Quantitative Analysis of Structural Isomers of Linear Pentaamines by GC–MS. Chromatographia 78, 723–727 (2015). https://doi.org/10.1007/s10337-015-2868-0

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  • DOI: https://doi.org/10.1007/s10337-015-2868-0

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