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In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS

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Abstract

Timosaponin BIII, as one of the steroid saponins isolated from Anemarrhena asphodeloides Bge., was proved to have many pharmacological activities in recent years and became a natural active compound with good development prospect. In the present study, a simple and rapid method using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry was developed for the determination of the structures of timosaponin BIII and its metabolites in rats after administrating intragastrically at 300 mg kg−1. By comparing their changes in molecular masses (ΔM), retention times and spectral patterns with those of the parent compound, nine metabolites were detected and identified in urine, and eight in plasma as well as four in brain. It is also indicated that the deglycosylation and oxidation reactions were the main metabolic pathways in the biotransformation of timosaponin BIII in vivo and the structures of the nine metabolites were identified and proposed to be timosaponin BII(M1), the hydroxylated metabolite of TBII(M2), the hydroxylated metabolites of TBIII(M3 and M4), deglycosylation and monooxygenation product of TBIII(M5), the deglycosylation product of TBII(M6), timosaponin AIII(M8), the isomers of timosaponin AIII(M7 and M9).

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 81030065 and No. 81274055).

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Authors and Affiliations

  1. Shanghai Institute of Materia Media, Chinese Academy of Sciences, Shanghai, 201203, China

    Dandan Li, Rui Xue, Zhixiong Li, Mingcang Chen & Chenggang Huang

  2. Harbin University of Commerce, Harbin, 150076, China

    Dandan Li & Weixin Jiang

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  1. Dandan Li
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  2. Rui Xue
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  3. Zhixiong Li
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  4. Mingcang Chen
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  5. Weixin Jiang
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  6. Chenggang Huang
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Correspondence to Weixin Jiang or Chenggang Huang.

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Li, D., Xue, R., Li, Z. et al. In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS. Chromatographia 77, 853–858 (2014). https://doi.org/10.1007/s10337-014-2681-1

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  • DOI: https://doi.org/10.1007/s10337-014-2681-1

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