Abstract
An efficient and simple method to isolate and purify highly polar antioxidants from the antioxidant active site of Chirita longgangensishas been established. Firstly, the antioxidant active site was enriched with D101 macroporous resin, and then high-speed counter-current chromatography (HSCCC) was used with the two-phase solvent system ethyl acetate–n-butanol–methanol–water (5:0.1:0.5:4.5, v/v) to obtain four antioxidants in one step. They were identified as plantainoside D (28.4 mg), plantainoside B (9.5 mg), calcedarioside B (18.1 mg) and calcedarioside A (16.7 mg) by analysis of electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) spectra. The purities were all above 97 % as determined by HPLC. The inhibiting effects of the crude extracts, enriched fraction and the obtained compounds on superoxide anion radical, hydroxyl radical and hydrogen peroxide were determined by different chemiluminescence (CL) systems. The result shows that all of them have good antioxidant activity. However, the sequence of antioxidant abilities among compounds I–IV was different when assayed by different CL systems (superoxide anion radical, hydroxyl radical, and hydrogen peroxide). This is the first report on preparative isolation and purification of antioxidants from C. longgangensis by HSCCC combined with macroporous resin and their inhibition of free radical-induced luminol chemiluminescence.
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Financial supports from the Natural Science Foundation of China (20872083, 21202094) the Science and Technology Program of ShanDong Academy of Sciences (201107), and Natural Science Foundation of Shandong Province (ZR2012HQ020).
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Duan, W., Bai, A., Lin, X. et al. Isolation and Purification of Highly Polar Antioxidants from Chirita longgangensis by Combination of Macroporous Resin and HSCCC. Chromatographia 77, 707–713 (2014). https://doi.org/10.1007/s10337-014-2665-1
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DOI: https://doi.org/10.1007/s10337-014-2665-1