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From Amino Acids Profile to Protein Identification: Searching for Differences in Roe Deer Papilloma

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Abstract

Papillomaviruses, small non-enveloped DNA viruses, are considered as the cause of a number of cancers, such as a cervical, skin, anal, and penile cancers. Roe deer papillomavirus infection can be easily characterized by typical warts on the skin. The aim of this study was to characterize differences of amino acid and protein composition in healthy and tumour tissue of roe deer using methods including ion-exchange chromatography, SDS-PAGE, two-dimensional gel electrophoresis and MALDI-TOF MS. The obtained data were statistically evaluated. Cluster analysis of all variables showed biggest differences in proline in control and papilloma samples. Further, we aimed at electrophoretic analysis of the samples. Discriminant analysis projections to latent structures (Projection to the latent structure Discrimination analysis, syn. Discrimination Partial least squares analysis, PLS DA) was used to evaluate the obtained results. Examining model PLS DA, there was found that the greatest effect on whether the individual is classified as healthy, or with papilloma, is the latent variable that best correlates with the molecular mass from 108.8 to 128.1 kDa and from 231.2 to 290.4 kDa in the original signal. Finally, using mass spectrometry three proteins, pyridoxal kinase, myoblast determination protein and leucine zipper transcription factor-like protein 1, most likely related to cancer were found.

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Acknowledgments

Financial support from CEITEC CZ.1.05/1.1.00/02.0068 and MSMT 6215712402 is highly acknowledged.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic - European Union

    Marketa Kominkova, Petr Michalek, Roman Guran, Natalia Cernei, Branislav Ruttkay-Nedecky, Ondrej Zitka, Vojtech Adam & Rene Kizek

  2. Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 616 00, Brno, Czech Republic - European Union

    Ondrej Zitka, Vojtech Adam & Rene Kizek

  3. Department of Cybernetics, Faculty of Electrical Engineering, Czech Technical University, Prague, Karlovo Namesti 13, 121 35, Prague 2, Czech Republic - European Union

    Jiri Anyz & Olga Stepankova

  4. Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 612 42, Brno, Czech Republic - European Union

    Ondrej Zitka, Jiri Pikula & Miroslava Beklova

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  1. Marketa Kominkova
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  2. Petr Michalek
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Correspondence to Rene Kizek.

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Kominkova, M., Michalek, P., Guran, R. et al. From Amino Acids Profile to Protein Identification: Searching for Differences in Roe Deer Papilloma. Chromatographia 77, 609–617 (2014). https://doi.org/10.1007/s10337-014-2658-0

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  • DOI: https://doi.org/10.1007/s10337-014-2658-0

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