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Determination of Fatty Acids in Saliva of Smokers and Nonsmokers by HPLC with Fluorescence Detection Using a Hydrazine-Based Difluoro-boraindacene Reagent

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Abstract

1,3,5,7-Tetramethyl-8-daminozide-difluoroboradiaza-s-indacence (TMBODIPY-H), a pre-column fluorescent derivatization reagent, was applied to the analysis of fatty acid by high-performance liquid chromatography with fluorescence detection. Using this reagent, 12 fatty acids from propionic acid (C3) to stearic acid (C18) can be derivatized at room temperature in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide within 2 h. The baseline separation of these derivatives can be achieved within 46 min by gradient elution. The detection limits of these fatty acids are in the range of 0.1–1 nM and the linear ranges of most of them are 0.5–100 nM. This was the first application of hydrazine-based difluoro-boraindacene reagent for the analysis of fatty acids, and the proposed method has been successfully used for the determination of saliva samples of smokers and nonsmokers with recoveries of 88–110 %.

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Acknowledgments

The research presented in this manuscript was supported by the National Natural Science Foundation of China (Nos. 20835004, 31170344 and 21105074, Beijing, China).

Conflict of interest

We declare that we have no financial or personal relationships with other people or organizations that can inappropriately influence our work.

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China

    Xiao-Feng Guo, Hong Wang & Hua-Shan Zhang

  2. Hubei Province Tobacco Quality Supervision and Test Station, Wuhan, 430030, China

    Yun Li

Authors
  1. Xiao-Feng Guo
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  2. Yun Li
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  3. Hong Wang
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  4. Hua-Shan Zhang
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Correspondence to Hong Wang.

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Guo, XF., Li, Y., Wang, H. et al. Determination of Fatty Acids in Saliva of Smokers and Nonsmokers by HPLC with Fluorescence Detection Using a Hydrazine-Based Difluoro-boraindacene Reagent. Chromatographia 77, 431–438 (2014). https://doi.org/10.1007/s10337-014-2627-7

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  • DOI: https://doi.org/10.1007/s10337-014-2627-7

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