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Determination of Metabolism of Neohesperidin by Human Intestinal Bacteria by UPLC-Q-TOF/MS

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Abstract

Neohesperidin as the major isoflavonoid in Aurantii Fructus has been investigated intensively. However, the route and metabolites of neohesperidin by human intestinal bacteria are not well understood and its metabolites may accumulate and exert physiological effects. In this work, 5 strains including Clostridium sp.8, Bacteroides sp.15, Bacillus sp.46, and Enterobacter sp.41-1 and sp.73 were isolated and their abilities to convert neohesperidin were studied. Human fecal microflora were prepared from a healthy Chinese woman and then anaerobically incubated with neohesperidin sample at 37 °C for 48 h. A rapid and simple liquid–liquid extraction method was used for sample pretreatment. A highly sensitive and specific ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry with MetaboLynx™ method has been developed for the analysis of neohesperidin and related metabolites in the reaction samples. The chromatographic separation was performed on a 1.7 μm particle size Syncronis C18 column using gradient elution system. The components in the extract were identified and confirmed according to the mass spectrometric fragmention mechanisms, MS/MS fragment ions and relevant literature by means of electrospray ionization mass spectrometry in negative ion mode. With this method, a total of five metabolites were detected, the results indicated that hydrolysis, dehydroxylation, demethylation and acetylation were the major metabolism of neohesperidin. The present study provided important information about the metabolism of neohesperidin which will be helpful for fully understanding the impact of the intestinal bacteria on this active component. Furthermore, this work demonstrated the potential of the ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry approach using MetaboLynx™ for quite rapid, simple, reliable and automated identification of metabolites of natural product.

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Acknowledgments

This work was financially supported by National Basic Research Program of China (973 Program) (2011CB505300, 2011CB505303); Key Research Project in Basic Science of Jiangsu College and University (10KJA360039); Construction Project for Jiangsu Key Laboratory for High Technology Research of TCM Formulae (BM2010576). We are also pleased to thank Waters China Ltd. for technical support.

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Authors and Affiliations

  1. Laboratory for High Technology Research of TCM Formulae, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing, 210046, People’s Republic of China

    Wei Zhang, Shu Jiang, Dawei Qian, Er-xin Shang & Jin-ao Duan

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  1. Wei Zhang
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  2. Shu Jiang
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  3. Dawei Qian
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  4. Er-xin Shang
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Correspondence to Wei Zhang.

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Zhang, W., Jiang, S., Qian, D. et al. Determination of Metabolism of Neohesperidin by Human Intestinal Bacteria by UPLC-Q-TOF/MS. Chromatographia 77, 439–445 (2014). https://doi.org/10.1007/s10337-014-2625-9

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  • DOI: https://doi.org/10.1007/s10337-014-2625-9

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