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Validated HPLC Method for Simultaneous Quantitation of Bergenin, Arbutin, and Gallic Acid in Leaves of Different Bergenia Species

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Abstract

Bergenia species (Saxifragaceae) are important sources of herbal medicines in Asia, mainly in Russia. Various plant parts are valued for their antibacterial, anti-inflammatory, antioxidant sand adaptogenic effect, and used for the dissolution of kidney and bladder stones. In this study a rapid reversed phase liquid chromatography (RP-HPLC) method has been developed for rapid screening and identifying of the main active components in leaf samples of Bergenia accessions. The main goal of this study was to develop an efficient method for the simultaneous identification and detection of arbutin, bergenin and gallic acid from Bergenia leaf samples, which were extracted with a methanolic solvent mixture [methanol:water = 1:1 (v/v)]. Chromatographic separations were performed on a reversed phase Luna C18(2)-HST HPLC column. This chromatographic system provided increased speed and efficiency for separations, without the need for ultra-high pressures. Reversed phase HPLC coupled with diode array detector method was used for the analysis. The method was validated using ICH guidelines. The level of gallic acid was significantly higher in Bergenia crassifolia samples compared to Bergenia cordifolia. However, the samples of the two Bergenia species did not differ substantially regarding the concentrations of arbutin and bergenin. The novel method proved to be fast and allowed sufficient separation and quantification of arbutin, bergenin and gallic acid, the most important bioactive compounds of Bergenia leaves; thus facilitating rapid screening and quality assessment of Bergenia samples of various botanical and geographical origins.

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References

  1. Harborne JB (1993) Phytochemistry 33:1279. doi:10.1016/0031-9422(93)85072-Y

  2. Shilova I, Pisareva S, Krasnov E, Bruzhes M, Pyak A (2006) Pharm Chem J 40:620–623

    Article  CAS  Google Scholar 

  3. Pozharitskaya ON, Ivanova SA, Shikov AN, Makarov VG, Galambosi B (2007) J Sep Sci 30:2447–2451. doi:10.1002/jssc.200700178

    Article  CAS  Google Scholar 

  4. Panossian A, Wikman G (2005) Arquivos Brasileiros de Fitomedicina Científica 2:109–131

    Google Scholar 

  5. Shikov AN, Pozharitskaya ON, Makarova MN, Dorman HJD, Makarov VG, Hiltunen R, Galambosi B (2010) J Funct Foods 2:71–76. doi:10.1016/j/jff.2009.11.003

    Article  Google Scholar 

  6. Shikov AN, Pozharitskaya ON, Dorman HJD, Makarov VG, Tikhonov VP, Hiltunen R (2007) Planta Med 73:897–897

    Google Scholar 

  7. Reddy UDC, Chawla AS, Deepak M, Singh D, Handa SS (1999) Phytochem Anal 10:44–47. doi:10.1002/(sici)1099-1565(199901/02)10:1<44:aid-pca424>3.0.co;2-4

    Article  CAS  Google Scholar 

  8. Singh DP, Srivastava SK, Govindarajan R, Rawat AKS (2007) Acta Chromatogr 19:246–252

    CAS  Google Scholar 

  9. Galambosi B, Galambosi Z, Siivari J (2009) Acta Hortic 860:129

    Google Scholar 

  10. Dharmender R, Madhavi T, Reena A, Sheetal A (2010) Pharmceutica Anal Acta 1:104

    CAS  Google Scholar 

  11. Swarnalakshmi T, Sethuraman MG, Sulochana N, Arivudainambi R (1984) Curr Sci 53:917–917

    Google Scholar 

  12. Lim HK, Kim HS, Choi HS, Oh S, Choi J (2000) J Ethnopharmacol 72:469–474. doi:10.1016/s0378-8741(00)00260-9

    Article  CAS  Google Scholar 

  13. Takahashi H, Kosaka M, Watanabe Y, Nakade K, Fukuyama Y (2003) Bioorg Med Chem 11:1781–1788. doi:10.1016/s0968-0896(02)00666-1

    Article  CAS  Google Scholar 

  14. Romm A, Yarnell E, Winston D (2010) Botanical medicine for women’s health. Elsevier, Amsterdam

    Google Scholar 

  15. Novak J (2010) Z Fur Arznei Gewurzpflanzen 15:170–173

    CAS  Google Scholar 

  16. Gallarate M, Carlotti ME, Trotta M, Grande AE, Talarico C (2004) J Cosmet Sci 55:139–148

    CAS  Google Scholar 

  17. Lin C-H, Wu H-L, Huang Y-L (2005) J Chromatogr B 829:149–152. doi:10.1016/j.jchromb.2005.10.008

    Google Scholar 

  18. Kittipongpatana N, Tangjai T, Srimanee A, Kittipongpatana O (2007) Chiang Mai Univ J 6:219

    Google Scholar 

  19. Pyka A, Bober K, Stolarczyk A (2007) Acta Pol Pharm 63:395–400

    Google Scholar 

  20. Lamien-Meda A, Lukas B, Schmiderer C, Franz C, Novak J (2009) Phytochem Anal 20:416–420. doi:10.1002/pca.1142

    Article  CAS  Google Scholar 

  21. Lukas B, Schmiderer C, Mitteregger U, Novak J (2010) Food Chem 121:185–190. doi:10.1016/j.foodchem.2009.12.028

    Article  CAS  Google Scholar 

  22. Pop C, Vlase L, Tamas M (2009) Not Bot Hortic Agrobot Cluj Napoca 37:129–132

    CAS  Google Scholar 

  23. Chauhan SK, Singh B, Agrawal S (2000) J AOAC Int 83:1480–1483

    CAS  Google Scholar 

  24. Srivastava S, Rawat AKS (2007) Jpc J Planar Chromatogr Modern Tlc 20:275–277. doi:10.1556/jpc.2007.6002

    Article  CAS  Google Scholar 

  25. Dhalwal K, Shinde VM, Biradar YS, Mahadik KR (2008) J Food Compos Anal 21:496–500. doi:10.1016/j.jfca.2008.02.008

    Article  CAS  Google Scholar 

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Acknowledgments

This research was realized in the frames of TÁMOP 4.2.4. A/2-11-1-2012-0001 “National Excellence Program—Elaborating and operating an inland student and researcher personal support system.” The project was subsidized by the European Union and co-financed by the European Social Fund. The work was supported by the grants OTKA K75717, SROP-4.2.2/08/1/2008-0011, and SROP-4.2.1.B-10/2/KONV-2010-0002. Dr. Tivadar Farkas (Phenomenex, Torrance, CA, USA) is acknowledged for the donation of the Luna 2.5 C18(2)-HST column used in this work.

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Authors and Affiliations

  1. Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6, Pécs, 7624, Hungary

    Borbála Boros, Tamás Madarász, Ferenc Kilár & Attila Felinger

  2. Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovakia

    Silvia Jakabová

  3. Department of Pharmacognosy, Medical School, University of Pécs, Pécs, Hungary

    Réka Molnár & Ágnes Farkas

  4. Agrifood Research Finland Mikkeli, Mikkeli, Finland

    Bertalan Galambosi

  5. Institute of Bioanalysis, Medical School, University of Pécs, Pécs, Hungary

    Ferenc Kilár

Authors
  1. Borbála Boros
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  2. Silvia Jakabová
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  3. Tamás Madarász
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  4. Réka Molnár
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  5. Bertalan Galambosi
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  6. Ferenc Kilár
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  7. Attila Felinger
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  8. Ágnes Farkas
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Correspondence to Attila Felinger.

Additional information

Published in the special paper collection 9th Balaton Symposium on High-Performance Separations Methods with guest editor Attila Felinger.

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Boros, B., Jakabová, S., Madarász, T. et al. Validated HPLC Method for Simultaneous Quantitation of Bergenin, Arbutin, and Gallic Acid in Leaves of Different Bergenia Species. Chromatographia 77, 1129–1135 (2014). https://doi.org/10.1007/s10337-014-2624-x

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  • DOI: https://doi.org/10.1007/s10337-014-2624-x

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