Skip to main content
SpringerLink
Log in

Volatile Organic Compounds Analysis in Breath Air in Healthy Volunteers and Patients Suffering Epidermoid Laryngeal Carcinomas

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Exhaled breath contains thousands of gaseous volatile organic compounds (VOCs) that may be used as non-invasive markers of head and neck epidermoid cancer. We hypothesized that solid phase micro-extraction coupled to gas chromatography–mass spectrometry can discriminate patients with epidermoid head and neck cancer from healthy controls by analyzing the gaseous volatile organic compounds, VOC-profile, in exhaled breath, thus identifying some non-invasive biomarkers to be used in early detection. Twenty healthy subjects participated in a cross-sectional study plus 11 patients with epidermoid supraglottic laryngeal cancer. VOCs from T3 supraglottic cancer were clustered distinctly from those of T1 and healthy subjects. Up to seven VOCs were detected differently from healthy volunteers, mainly 2-butanone and ethanol. Thus VOC-patterns of exhaled breath may discriminate patients with epidermoid head and neck cancer from healthy controls.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Bezerra DL, Bernal MM, Curado MP (2011) Cancer Epidemiol 35:328–333

    Article  Google Scholar 

  2. Maroni G, Ragona RM, Cartei G, Marchese F, Staffieri A (2006) Cancer Treat Rev 32:504–515

    Article  Google Scholar 

  3. Cattaruzza MS, Maisonneuve P, Boyle P (1996) Eur J Cancer 32:293–305

    Article  Google Scholar 

  4. Bajtarevic A, Ager C, Pienz M, Klieber M, Schwarz K, Ligor M (2009) BMC Cancer 348:471–479

    Google Scholar 

  5. Hughes C, Homer J, Bradley P, Nutting C, Ness A, Persson M, Jeffreys M, Waylen A, Leary S, Thomas S (2012) Clin Oncol 24:187–192

    Article  Google Scholar 

  6. Tong CCL, Kao J, Sikora AG (2012) Immunol Res 54:266–274

    Article  CAS  Google Scholar 

  7. Phillips M, Cataneo RN, Cummin ARC, Gagliardi AJ, Gleeson K, Greenberg J (2003) Am Coll Chest Physicians 123:2115–2123

    CAS  Google Scholar 

  8. Phillips M, Gleeson K, Hughes JMB, Greenberg J, Cataneo RN, Baker L (1999) Lancet 353:1930–1942

    Article  CAS  Google Scholar 

  9. Phillips M (1992) Sci Am 267:74–79

    Article  CAS  Google Scholar 

  10. Gordon SM, Szidon SP, Krotoszynski BK, Gibbons RD, O’Neill HJ (1985) Clin Chem 31:1278–1289

    CAS  Google Scholar 

  11. Pauling L, Robinson AB, Teranishi R, Gary P (1971) Proc Natl Acad Sci USA 68:2374–2376

    Article  CAS  Google Scholar 

  12. O’Neill HJ, Gordon SM, O’Neill MH, Gibbons RD, Szidon JP (1988) Clin Chem 34:1613

    Google Scholar 

  13. Damas MJ, Yonamine M, de Moraes RL, Silva AO (2004) J Chromatogr B 811:37–40

    Article  Google Scholar 

  14. Kataoka H, Saito K (2011) J Pharmaceut Biomed 54:926–950

    Article  CAS  Google Scholar 

  15. Hakim M, Broza YY, Barash O, Peled N, Phillips M, Amann A, Hossam H (2012) Chem Rev 112:5949–5966

    Article  CAS  Google Scholar 

  16. Buszewski B, Kesy M, Ligor T, Amann A (2007) Biomed Chromatogr 21:553–566

    Article  CAS  Google Scholar 

  17. Kataoka H (2010) Anal Bioanal Chem 396:339–364

    Article  CAS  Google Scholar 

  18. Theodoridos G, Koster EHM, Jong GJ (2000) J Chromatogr B 745:49–82

    Article  Google Scholar 

  19. Arthur C, Pawliszyn J (1990) Anal Chem 62:2145–2148

    Article  CAS  Google Scholar 

  20. Wang VS, Lu MY (2009) J Chromatogr B 877:24–32

    Article  CAS  Google Scholar 

  21. Górecki T, Yu X, Pawliszyn J (1999) Analyst 124:643–649

    Article  Google Scholar 

  22. Ligor T, Szeliga J, Jackowski M, Buszewski B (2007) J Breath Res 1:016001

    Article  CAS  Google Scholar 

  23. Buszewski B, Ulanowska A, Kowalkowski T, Cieslinski K (2012) Clin Chem Lab Med 50(3):573–581

    Article  CAS  Google Scholar 

  24. DeGreeff LE, Furton KG (2011) Anal Bioanal Chem 401:1295–1307

    Article  CAS  Google Scholar 

  25. Pandey SK, Kim KH (2010) Trends Anal Chem 29:804–819

    Article  CAS  Google Scholar 

  26. Phillips M (1997) Anal Biochem 247:272–278

    Article  CAS  Google Scholar 

  27. Díaz Maroto MC, Sánchez Palomo E, Pérez Coello MS (2004) J Agric Food Chem 52:6857–6861

    Article  Google Scholar 

  28. Mochalski P, King J, Unterkoflera K, Amannad A (2013) Analyst 138:1405–1418

    Article  CAS  Google Scholar 

  29. Ghosh S, Kim K-H, Sohn JR (2011) Sci World J 11:2160–2177

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank all the participants in our study, especially those who are suffering from cancer. The financial support of Fundación Mútua Madrileña and the Spanish government (CTQ2008-05909/PPQ and CTQ2011-22707 projects) is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Department of Chemical and Environmental Technology, Rey Juan Carlos University, C/ Tulipán s/n, 28933, Móstoles, Madrid, Spain

    Rafael A. García, Victoria Morales, Sergio Martín & Estela Vilches

  2. Department of Otorhinolaryngology, Foundation Hospital Alcorcón, C/Budapest 1, 28922, Alcorcón, Spain

    Adolfo Toledano

Authors
  1. Rafael A. García
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Victoria Morales
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sergio Martín
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Estela Vilches
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Adolfo Toledano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rafael A. García.

Rights and permissions

Reprints and permissions

About this article

Cite this article

García, R.A., Morales, V., Martín, S. et al. Volatile Organic Compounds Analysis in Breath Air in Healthy Volunteers and Patients Suffering Epidermoid Laryngeal Carcinomas. Chromatographia 77, 501–509 (2014). https://doi.org/10.1007/s10337-013-2611-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-013-2611-7

Keywords

Search

Navigation