Skip to main content
SpringerLink
Log in

Deep Eutectic Solvent-Based HS-SME Coupled with GC for the Analysis of Bioactive Terpenoids in Chamaecyparis obtusa Leaves

  • Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Headspace-solvent microextraction (HS-SME) was developed as a solvent-minimized extraction technique, but few studies have examined the applications of deep eutectic solvents (DESs) to the HS-SME of bioactive compounds. In this study, HS-SME was developed for the extraction of bioactive compounds using DESs as extraction solvents. DESs, which were prepared by mixing choline chloride (ChCl) with ethylene glycol (EG) at different ratios, were applied to the extraction of three terpenoids from Chamaecyparis obtusa leaves by HS-SME. The ChCl/EG ratio in the DESs, HS-SME conditions, such as the extraction temperature and extraction time, and sample/DES ratio were optimized. All extracts were analyzed by GC. Under optimized conditions, the limits of detection were 2.006 ng mL−1 for linalool, 3.150 ng mL−1 for α-terpineol and 2.129 ng mL−1 for terpinyl acetate. The relative standard deviations were in the range of 2.1–6.8 %. The recoveries of the three terpenoids were in the range of 79.4–103 %. HS-SME is simple and rapid compared to heat reflux extraction and ultrasonic extraction. Moreover, DESs can be used in HS-SME for the extraction of a range of bioactive and volatile compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Theis AL, Waldack AJ, Hansen SM, Jeannot MA (2001) Headspace solvent microextraction. Anal Chem 73:5651–5654

    Article  CAS  Google Scholar 

  2. Xu L, Basheer C, Lee HK (2007) Developments in single-drop microextraction. J Chromatogr A 1152:184–192

    Article  CAS  Google Scholar 

  3. Yao C, Twu P, Anderson JL (2010) Headspace single drop microextraction using micellar ionic liquid extraction solvents. Chromatographia 72:393–402

    Article  CAS  Google Scholar 

  4. Zhang J, Lee HK (2010) Headspace ionic liquid-based microdrop liquid-phase microextraction followed by microdrop thermal desorption-gas chromatographic analysis. Talanta 81:537–542

    Article  Google Scholar 

  5. Zhang Q, Vigier KDO, Royer S, Jérôme F (2012) Deep eutectic solvents: syntheses, properties and applications. Chem Soc Rev 41:7108–7146

    Article  CAS  Google Scholar 

  6. Abbott A, Boothby B, Capper G, Davis D (2004) Deep eutectic solvents formed between choline chloride and carboxylic acids: versatile alternatives to ionic liquids. J Am Chem Soc 126:9142–9147

    Article  CAS  Google Scholar 

  7. Hong EJ, Na KJ, Choi IG, Choi KC, Jeung EB (2004) Antibacterial and antifungal effects of essential oils from coniferous trees. Biol Pharm Bull 27:863–866

    Article  CAS  Google Scholar 

  8. Marimuthu P, Wu CL, Chang HT, Chang ST (2008) Antioxidant activity of the ethanolic extract from the bark of Chamaecyparis obtusa var. formosana. J Sci Food Agric 88:1400–1405

    Article  CAS  Google Scholar 

  9. Barrero AF, Quilez del Moral JF, Herrador MM, Akssira M, Bennamara A, Akkad S, Aitigri M (2004) Oxygenated diterpenes and other constituents from Moroccan Juniperus phoenicea and Juniperus thurifera var. africana. Phytochemistry 65:2507–2515

    Article  CAS  Google Scholar 

  10. Okasaka M, Takaishi Y, Kashiwada Y, Kodzhimatov O, Ashurmetov O, Lin AJ, Consentino LM, Lee KH (2006) Terpenoids from Juniperus polycarpus var. seravschanica. Phytochemistry 67:635–2640

    Article  Google Scholar 

  11. Bicchi C, Cordero C, Liberto E, Sgorbini B, Rubiolo P (2008) Headspace sampling of the volatile fraction of vegetable matrices. J Chromatogr A 1184:220–233

    Article  CAS  Google Scholar 

  12. Shen G, Lee HK (2003) Headspace liquid-phase microextraction of chlorobenzenes in soil with gas chromatography-electron capture detection. Anal Chem 75:98–103

    Article  CAS  Google Scholar 

  13. Tankeviciute A, Kazlauskas R, Vickackaite V (2001) Headspace extraction of alcohols into a single drop. Analyst 126:1674–1677

    Article  CAS  Google Scholar 

  14. Smith WL (1977) Selective solubility: “like dissolves like”. J Chem Educ 54:228–229

    Article  CAS  Google Scholar 

  15. Yan X, Yang C, Ren C, Li D (2008) Importance of extracting solvent vapor pressure in headspace liquid-phase microextraction. J Chromatogr A 1205:182–185

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (No. 2011-0010673).

Author information

Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, Inha University, Incheon, 402-751, Korea

    Baokun Tang, Wentao Bi, Heng Zhang & Kyung Ho Row

Authors
  1. Baokun Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wentao Bi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Heng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kyung Ho Row
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kyung Ho Row.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, B., Bi, W., Zhang, H. et al. Deep Eutectic Solvent-Based HS-SME Coupled with GC for the Analysis of Bioactive Terpenoids in Chamaecyparis obtusa Leaves. Chromatographia 77, 373–377 (2014). https://doi.org/10.1007/s10337-013-2607-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-013-2607-3

Keywords

Search

Navigation