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Simultaneous Determination of Nelarabine and Its Active Metabolite 9-β-d-Arabinofuranosylguanine (Ara-G) in Human Plasma

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Abstract

A novel, simple and reliable bioanalytical method of high performance liquid chromatography for simultaneous quantification of nelarabine and its active metabolite 9-β-d-arabinofuranosyl-guanine (Ara-G) in human plasma was developed and validated. The calibration curves for nelarabine and Ara-G were linear over concentration ranges of 0.1562–10 and 0.6250–40 μg mL−1, respectively. Intra- and inter-day precision and accuracy results satisfied the acceptable criteria for bioanalytical method validation. This method has been applied to a pharmacokinetic study involving 20 T-cell lymphoblastic leukemia/lymphoma patients.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Beijing, China) for Project No. 81102499 which was titled as “Kinetic study on intra-cellular phosphorylation of nucleoside analogs”, and supported by the Fundamental Research Funds for the Central Universities of Central South University and Hunan Science and Technology Project (No. 2011SK3261).

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Central South University, Changsha, 410013, China

    Xingling Liu, Sanwang Li, Zeneng Cheng, Feifan Xie & Peng Yu

  2. Bioanalytical Laboratory, Hunan Tiger-Xiangya R&D Company Ltd., Changsha, 410013, China

    Hang Cheng, Zhi Liu & Xin Guo

Authors
  1. Xingling Liu
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  2. Sanwang Li
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  3. Zeneng Cheng
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  4. Hang Cheng
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  5. Zhi Liu
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  6. Xin Guo
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  7. Feifan Xie
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  8. Peng Yu
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Corresponding author

Correspondence to Peng Yu.

Additional information

X. Liu and S. Li have contributed equally to this work.

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Liu, X., Li, S., Cheng, Z. et al. Simultaneous Determination of Nelarabine and Its Active Metabolite 9-β-d-Arabinofuranosylguanine (Ara-G) in Human Plasma. Chromatographia 77, 91–97 (2014). https://doi.org/10.1007/s10337-013-2581-9

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  • DOI: https://doi.org/10.1007/s10337-013-2581-9

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