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Characterization of the Interaction Between Lantibiotics, Nisin and Duramycin, and β-Lactoglobulin by Affinity Capillary Electrophoresis

Chromatographia volume 76, pages 1773–1777 (2013)Cite this article

Abstract

Affinity capillary electrophoresis was used to study quantitatively the noncovalent interactions between β-lactoglobulin (β-LG), a milk whey protein, and two lantibiotics, nisin (a dairy biopreservative lantibiotic) and duramycin (a bovine mastitis treatment lantibiotic). The study involved measuring the change in effective electrophoretic mobility of the lantibiotic as the concentration of β-LG in the background electrolyte is increased. Nonlinear regression analysis was used to model the dependence of the effective mobility of the lantibiotic on β-LG concentration in the BGE. Using this approach, binding constants were determined to be 3.1 (±0.2) × 108 M−1 for nisin and 2.2 (±0.1) × 108 M−1 for duramycin. Both binding constants were comparable indicating the similarity of affinity properties of nisin and duramycin towards β-LG. These results demonstrate that affinity capillary electrophoresis is a suitable method for characterizing the interaction between lantibiotics and β-LG.

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References

  1. Kontopidis G, Holt C, Sawyer L (2004) J Dairy Sci 87:785–796

    Article  CAS  Google Scholar 

  2. Qin BY, Bewley MC, Creamer LK, Baker HM, Baker EN, Jameson GB (1999) Prot. Sci. 8:75–83

    Article  CAS  Google Scholar 

  3. Pérez MD, Calvo M (1995) J Dairy Sci 78(5):978–988

    Article  Google Scholar 

  4. Jung G (1996) Combinatorial Peptides and Nonpeptide Libraries. VCH: Weinheim, Germany: 303

  5. Järvinen K-M, Chatchatee P, Bardina L, Beyer K, Sampson HA (2001) Int. Arch. Of Allergy and Immun. 126(2):111–118

    Article  Google Scholar 

  6. Willey JM, van der Donk WA (2007) Annu Rev Microbiol 61:477–501

    Article  CAS  Google Scholar 

  7. Twomey D, Ross RP, Meaney B, Jill C (2002) Antoine van Leeuwenhoek. 82:165–185

    Article  CAS  Google Scholar 

  8. Field D, Connor PMO, Cotter PD, Hill C, Ross RP (2008) Mol Micro 69(1):218–230

    Article  CAS  Google Scholar 

  9. de Arauz LJ, Jozala AF, Mazzola PG, Penna TCV (2009) Trends Food Sci Technol 20:146–154

    Article  Google Scholar 

  10. Delves-Broughton J (2005) Food Australia 57(12):525–527

    CAS  Google Scholar 

  11. Chen H, Hoover DG (2003) Comp Revs Food Sci Food Saf 2:82–100

    CAS  Google Scholar 

  12. Cotter PD, Hill C, Ross PR (2005) Curr Prot Pep Sci 6:61–75

    Article  CAS  Google Scholar 

  13. Yada RY (2004) Proteins in food processing CRC Press, Boca Raton 402

  14. El Deeb S, Wätzing H, El-Hady A (2013) Trends in. Anal Chem 48:112–131

    CAS  Google Scholar 

  15. Bowser MT, Chen DDY (1998) Anal Chem 70:3261–3270

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Natural Science and Engineering Research Council (NSERC) of Canada for funding this study and Thompson Rivers University (TRU) for their support.

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Authors and Affiliations

  1. Department of Chemistry, Thompson Rivers University, 900 Mc Gill Road, Kamloops, BC, V2C 0C8, Canada

    Jessica M. Risley, Laiel C. Soliman & Kingsley K. Donkor

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  1. Jessica M. Risley
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  2. Laiel C. Soliman
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  3. Kingsley K. Donkor
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Correspondence to Kingsley K. Donkor.

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Risley, J.M., Soliman, L.C. & Donkor, K.K. Characterization of the Interaction Between Lantibiotics, Nisin and Duramycin, and β-Lactoglobulin by Affinity Capillary Electrophoresis. Chromatographia 76, 1773–1777 (2013). https://doi.org/10.1007/s10337-013-2579-3

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  • DOI: https://doi.org/10.1007/s10337-013-2579-3

Keywords

  • Capillary electrophoresis
  • Lantibiotics
  • Nisin
  • Duramycin
  • β-Lactoglobulin
  • Binding constant