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LC–MS Based Workflows for Qualitative and Quantitative Analysis for Homeopathic Preparation of Hydrastis canadensis

Abstract

Hydrastis canadensis L. is one of the important herbal medicinal plants, which is used for homeopathic preparations. It contains berberine, hydrastine as major and canadine, hydrastinine, berberastine and canadaline as minor bioactive compounds. The aim was to develop (I) a sensitive mass spectrometric-based quantitative/qualitative method for berberine in homeopathic dilutions of H. canadensis, (II) with identification of berberine and its structurally related compound present in the homeopathic preparation of H. canadensis using predictive MRM workflow, (III) confirmatory analysis for the structurally related compound in homeopathic dilutions of H. canadensis using high-resolution mass spectrometer. Different mass spectrometric-based workflows (MRM to EPI; pMRM to EPI and IDA-DBS) were used to develop the method to analyze the homeopathic dilutions of H. canadensis. A high-throughput liquid chromatography-tandem mass spectrometry method using MRM to EPI workflow was developed to analyze berberine in homeopathic preparation of H. canadensis. Multiple reaction monitoring transitions of 336.0/292.08 and 336.0/277.9 were selected for developing the quantitation method of berberine. The method was linear from 5.0 to 2000.0 pg/mL. The validated method has been successfully applied for the analysis of different potencies of homeopathic preparation of H. canadensis. Predictive MRM to enhanced product ion workflows was used to identify and confirm the structurally related molecules of berberine. Non targeted information dependent acquisition-DBS method was developed to identify the compounds in H. canadensis mother tincture using high-resolution mass spectrometry. The established method using different mass spectrometric workflows facilitates the convenient and fast quality control analysis for the herbal preparation of H. canadensis.

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References

  1. 1.

    Weber HA, Zart MK, Fergusson SL, Greaves JG, Clark AP, Harris RK, Overstreet D, Smith C (2001) J Liq Chrom Rel Technol 24(1):87–95

    CAS  Article  Google Scholar 

  2. 2.

    Weber HA, Zart MK, Molloy HM, Brien BMO, Moody LA, Clark AP, Harris RK, Overstreet D, Smith C (2003) J Agric Food Chem 51:7352–7360

    CAS  Article  Google Scholar 

  3. 3.

    Blumental M (1999) Herbalgram 47:64–65

    Google Scholar 

  4. 4.

    United States Pharmacopeial Convention: USP 26-NF21 Supplement 1 2003

  5. 5.

    Rojsanga P, Gritsanapan W, Suntornsuk L (2006) Med Princ Pract 15:373–378

    Article  Google Scholar 

  6. 6.

    Chen YR, Wen KC, Her GR (2000) J Chromatogr A 866:273–280

    CAS  Article  Google Scholar 

  7. 7.

    Suto K, Kakinuma S, Ito Y, Sagara K, Iwasaki H, Itokawa H (1997) J Chromatogr A 786:371–376

    CAS  Article  Google Scholar 

  8. 8.

    Chattopadhyay SK, Kumar S (2007) Biomed Chromatogr 21:55–66

    CAS  Article  Google Scholar 

  9. 9.

    Zhang Y, Wu W, Han F, Chen Y (2008) Biomed Chromatogr 22:1360–1367

    CAS  Article  Google Scholar 

  10. 10.

    Yang KY, Lin LC, Tseng TY, Wang SC, Tsai TH (2007) J Chromatogr A 853:183–189

    CAS  Google Scholar 

  11. 11.

    Gratz SR, Flurer CL, Wolnik KA (2004) J Pharm Biomed Anal 36:525–533

    CAS  Article  Google Scholar 

  12. 12.

    Lee MC, Tsao CH, Iou SC, Chuang WC, Sheu SJ (2003) J Sep Sci 26:818–822

    CAS  Article  Google Scholar 

  13. 13.

    Hua W, Ding L, Chen Y, Gong B, He J, Xu G (2007) J Pharm Biomed Anal 44:931–937

    CAS  Article  Google Scholar 

  14. 14.

    Lu T, Liang Y, Song J, Deng Y, Xie L, Wang J, Liu X (2006) J Pharm Biomed Anal 40:1218–1222

    CAS  Article  Google Scholar 

  15. 15.

    Deng Y, Liao Q, Li S, Bi K, Pan B, Xie Z (2008) J Chromatogr B Analyt Technol Biomed Life Sci 863:195–205

    CAS  Article  Google Scholar 

  16. 16.

    Zuo F, Zhou ZM, Zhang Q, Mao D, Xiong YL, Wang YL, Yan MZ, Liu ML (2003) Biol Pharm Bull 26:911–915

    CAS  Article  Google Scholar 

  17. 17.

    Guidance for Industry: Bioanalytical method validation (2001) U.S. Department of Health and Human services food and drug administration center for drug evaluation and research (CDER) center of veterinary medicine (CVM)

  18. 18.

    Brown PN, Paley LA, Roman MC, Chan M (2008) Pharma Biol 46:135–139

    CAS  Article  Google Scholar 

  19. 19.

    Brown PN, Roman MC (2008) J AOAC Int 91:694–701

    CAS  Google Scholar 

  20. 20.

    Luo X, Chen B, Yao S (2005) Talanta 66:103–106

    CAS  Article  Google Scholar 

  21. 21.

    Hopfgartner G, Varesio E, Tschappat V, Grivet C, Bourgogne E, Leuthold LA (2004) J Mass Spectrom 39:845–855

    CAS  Article  Google Scholar 

  22. 22.

    ICH Q2B (1996) Validation of analytical procedure: methodology, international conference on harmonization of technical requirements for registration of pharmaceuticals for human use

  23. 23.

    Gupta PK, Hubbard M, Gurley B, Hendrickson HP (2009) J Pharm Biomed Anal 49:1021–1026

    CAS  Article  Google Scholar 

  24. 24.

    Zuo F, Nakamura N, Akao T, Hattori M (2006) Drug Metab Dispos 34:2064–2072

    CAS  Article  Google Scholar 

  25. 25.

    Carmai S (2010) ABSCIEX™ Tech note 1

  26. 26.

    Liu HF, Jones E (2008) AB SCIEX™ Tech note1

  27. 27.

    Tims MC (2006) Digital Repository at the University of Maryland. http://drum.lib.umd.edu/handle/1903/4052

  28. 28.

    Guillarme D, Schappler J, Rudaz S, Lveuthey J (2010) Trends Anal Chem 29:15–27

    CAS  Article  Google Scholar 

  29. 29.

    Shawn MR, Hsiao S, Foo C (2006) J Chromatogr B 836:1–14

    Article  Google Scholar 

  30. 30.

    Mueller CA, Weinmann W, Dresen S, Schreiber A, Gergov M (2005) Rapid Commun Mass Spectrum 19:1332–1338

    CAS  Article  Google Scholar 

  31. 31.

    Yves Le Blanc JC, Bloomfiled N (2004) Presented in ASMS, TPM, p 224

  32. 32.

    Bradley P (1992) British herbal compendium vol. 1. Bournemouth, UK: BHMA

  33. 33.

    Galeffi C, Cometa MF, Tomassini L, Nicoletti M (1997) Planta Med 63:194–195

    CAS  Article  Google Scholar 

  34. 34.

    Govindan M, Govindan G (2000) Fitoterapia 71:232–235

    CAS  Article  Google Scholar 

  35. 35.

    Upton R (2001) American Herbal Pharmacopoeia

  36. 36.

    Hwang BY, Roberts SK, Chadwick LR, Wu CD, Kinghorn AD (2003) Planta Med 69:623–627

    CAS  Article  Google Scholar 

  37. 37.

    Wang CZ, Kim KE, Du GJ, Qi LW, Wen XD, Li P, Bauer BA, Bissonnette M, Musch MW, Chang EB, Yuan CS (2011) Am J Chin Med 39:1161–1171

    CAS  Article  Google Scholar 

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Correspondence to Manoj G. Pillai.

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Pillai, M.G., Kumar, A., Sharma, R. et al. LC–MS Based Workflows for Qualitative and Quantitative Analysis for Homeopathic Preparation of Hydrastis canadensis . Chromatographia 77, 119–131 (2014). https://doi.org/10.1007/s10337-013-2577-5

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Keywords

  • High-throughput liquid chromatography-tandem mass spectrometry
  • Predictive MRM
  • Hydrastis canadensis
  • Homeopathic preparation
  • Berberine