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Simultaneous Determination of Ten Nucleosides and Related Compounds by MEEKC with [BMIM]PF6 as Oil Phase

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Abstract

In the present study, four nucleobases (adenine, cytosine, uracil, thymine), four nucleosides (adenosine, cytidine, uridine, thymidine), and two nucleotides (adenosine-5′-monophosphate, and cytidine-5′-monophosphate) were simultaneously determined by MEEKC with ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF6) as oil phase. Experimental parameters including the microemulsion compositions (surfactant, co-surfactant, and oil phase), pH, and concentration of borate buffer were intensively investigated. Finally, the ten compounds were well separated within 11 min using the running buffer composed of 140 mM SDS, 1.8 M n-butanol, and 10 mM [BMIM]PF6 in 20 mM borate buffer of pH 9.0. The developed method was successfully applied to determine the contents of investigated compounds in three different widely used traditional Chinese medicines (cultured Cordyceps sinensis, Radix Astragali, and Radix Isatidis). The results indicated that the developed MEEKC method could be used for the rapid determination of nucleobases, nucleosides, and nucleotides in herbal medicines or other complex matrices.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21175159, 21275169 and 81202886), the International Cooperation Project of Ministry of Science and Technology (2010DFA32680), and the Fundamental Research Funds for the Central Universities (No. CDJXS11232242).

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  1. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400030, China

    Feng Li, Feng-Qing Yang & Zhi-Ning Xia

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  1. Feng Li
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Correspondence to Zhi-Ning Xia.

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Li, F., Yang, FQ. & Xia, ZN. Simultaneous Determination of Ten Nucleosides and Related Compounds by MEEKC with [BMIM]PF6 as Oil Phase. Chromatographia 76, 1003–1011 (2013). https://doi.org/10.1007/s10337-013-2507-6

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