Abstract
Fragment-imprinted microspheres (FIMs) were prepared by suspension polymerization. 2,6-Dichloropyrimidine was used as the template molecule instead of sulfonamides, methacrylic acid and styrene were the mixed functional monomers, ethylene dimethacrylate was the crosslinking agent, and azodiisobutyronitrile was the initiator. The optimum conditions were obtained by an orthogonal experiment. The maximum adsorption was obtained under the optimum conditions. The adsorption by FIMs was 7.02 mg g−1, whereas the adsorption by non-FIMs was 5.41 mg g−1. A method for the simultaneous determination of five sulfonamides from milk samples by fragment imprinted-solid phase extraction coupled with HPLC was developed. The recoveries for the five sulfonamide extractions were 89.7–97.6 % with relative standard deviations of 1.79–3.02 %, indicating the suitability of the prepared FIMs for sulfonamide extraction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adrian J, Pasche S, Diserens JM, Sánchez-Baeza F, Gao H, Marco MP, Voirin G (2009) Biosens Bioelectron 24:3340–3346
Hyun-Hee C, Jung-Bin L, Yun-Hee C, Kwang-Geun L (2009) Food Chem 113:297–301
Sheridan R, Policastro B, Thomas S, Rice D (2008) J Agric Food Chem 56(10):3509–3516
Feng XQ, Meng GW (2012) Adv Mater Res 554–556:1909–1912
Chen L, Zhang X, Sun L, Xu Y, Zeng Q, Wang H, Xu H, Yu A, Zhang H, Ding L (2009) J Agric Food Chem 57(21):10073–10080
Gao R, Zhang J, He X, Chen L, Zhang Y (2010) Anal Bioanal Chem 398(1):451–461
Ben W–W, Qiang Z-M, Craig A, Zhang H-Q, Chen L-P (2008) J Chromatogr A 1202:173–180
Zhang H-Y, Wang S (2009) J Immunol Methods 350:1–13
Roybal JE, Pfenning AP, Turnipseed SB, Gonzales SA (2003) Ana Chim Acta 483:147–152
He J, Lv R-H, Cheng J, Li Y-X, Xue J-F, Lu K, Wang F-C (2010) J Sep Sci 33:3409–3414
Sharma PS, Souza FD, Kutner W (2012) TrAC-Trend Anal Chem 34:59–77
He J, Lv R-H, Zhan H-J, Wang H-Z, Cheng J, Lu K, Wang F-C (2010) Ana Chim Acta 674:53–58
Lok CM, Son R (2009) Food Res Int 16:127–140
He J, Shen Y-Z, Chen S, Wei H-L, Zhu J, You L-Q, Lu K (2010) J Sep Sci 33:3409–3414
Shi X-Z, Meng Y, Liu J-H, Sun A-L, Li D-X, Yao C-X, Lu Y, Chen J (2011) J Chromatogr B 879:1071–1076
Xu Z-G, Du Z, Hu Y-L, Hu Y-F, Pan Y-P, Li G-K (2012) Chin J Anal Chem 40(7):1002–1010
Kong X, Gao R-X, He X-W, Chen L-X, Zhang Y-K (2012) J Chromatogr A 1245:8–16
Shi X-Z, Meng Y, Liu J-H, Sun A-L, Li D-X, Yao C-X, Lu Y, Chen J (2011) J Chromatogr B 879:1071–1076
Theodoridis G, Kantifes A, Manesiotis P, Raikos N, Tsoukali-Papadopoulou H (2003) J Chromatogr A 987:103–109
Takuya K, Ken H, Yoshiyuki W, Tohru I, Nobuo T, Tomoharu S, Kunimitsu K (2004) J Chromatogr A 1029:37–41
Takuya K, Makoto N, Koji N, Tomoharu S, Ken H, Nobuo T, Kunimitsu K (2006) Ana Chim Acta 577:1–7
Takuya K (2008) Chromatogr 29:9–17
He J-F, Zhu Q-H, Deng Q-Y (2007) Spectrochim Acta A 67:1297–1305
Acknowledgments
The authors gratefully appreciate the financial support from the Key Scientific and Technological Project of Henan Province (112102310030), Natural Science Basic Research Plan in the Education Department of Henan Province (2010A150006), the National Natural Science Foundation of China (21275039) and Postgraduate Education Innovation Foundation of Henan University of Technology (11YJCX08).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
He, J., Tang, H., You, L. et al. Synthesis of Fragment-Imprinted Microspheres of 2,6-Dichloropyrimidine as Templates and Determination of Sulfonamides in Milk Samples. Chromatographia 76, 959–965 (2013). https://doi.org/10.1007/s10337-013-2500-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-013-2500-0