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Determination of Sulfonamides in Pharmaceuticals and Rabbit Plasma by Microchip Electrophoresis with LED-IF Detection

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Abstract

In this paper, we describe a compact and low-cost light-emitting diode-induced fluorescence (LED-IF) detection coupled to microchip electrophoresis for the determination of sulfonamides in pharmaceutical formulations and rabbit plasma. Three fluorescein isothiocyanate-labeled sulfonamides in rabbit plasma were separated in the running buffer of 40 mM phosphate buffer (pH 7.0) at the separation voltage of 2.0 kV, and detected by LED-IF detector in which the high-power blue LED was driven at the constant current of 150 mA and the emitted fluorescence over 510 nm was collected by a planar photodiode. The linear concentration ranged from 2.0 to 125.0 μg mL−1, both for sulfadiazine and sulfamethazine with the correlation coefficients (r 2) of 0.995 and 0.997, respectively, and from 2.0 to 100.0 μg mL−1 with the correlation coefficients (r 2) of 0.997 for sulfaguanidine. The limits of detection for the three sulfonamides were 0.36–0.50 μg mL−1 (S/N = 3). Intra-day and inter-day precision of migration time and peak area for the determination of sulfonamides were <4.5 %. This method has been successfully applied to the analysis of sulfonamides in pharmaceuticals, and could be used to study the pharmacokinetics of sulfonamides in rabbit.

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Acknowledgments

Financial supports from the National Natural Science Foundation of China (No. 20727006) and Guangdong Provincial Science and Technology Project (No. 2008A030102009) are gratefully acknowledged.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Sun Yat-sen University, 132 Waihuan East Road of Higher Education Mega Centre, Guangzhou, 510006, People’s Republic of China

    Beibei Zhang, Zuanguang Chen, Yanyan Yu, Jianping Yang & Jianbin Pan

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  1. Beibei Zhang
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  2. Zuanguang Chen
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  3. Yanyan Yu
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  4. Jianping Yang
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  5. Jianbin Pan
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Correspondence to Zuanguang Chen.

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Zhang, B., Chen, Z., Yu, Y. et al. Determination of Sulfonamides in Pharmaceuticals and Rabbit Plasma by Microchip Electrophoresis with LED-IF Detection. Chromatographia 76, 821–829 (2013). https://doi.org/10.1007/s10337-013-2479-6

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  • DOI: https://doi.org/10.1007/s10337-013-2479-6

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