Abstract
A fluorogenic derivatization method for the determination of chlorpropamide in human serum was developed by means of high-performance liquid chromatography (HPLC) with fluorescence detection. The Suzuki coupling reaction with a non-fluorescent reagent, phenylboronic acid (PBA), was employed to convert chlorpropamide into highly fluorescent biphenyl derivative. Chlorpropamide was extracted from human serum by liquid–liquid extraction with toluene after addition of hydrochloric acid, and subsequently reacted with PBA. Because the fluorogenic derivatization was highly selective for aryl halide, the proposed method allowed sensitive and selective detection of chlorpropamide with a detection limit (at a signal to noise ratio of 3) of 0.5 ng mL−1. The sensitivity of our method was from 4 to 100 times better than HPLC–UV, gas chromatography, and LC-mass spectrometry.
References
Diehl AK, Sugarek NJ, Bauer RL (1985) Diabetes Care 8:219–223
Klonoff DC (1988) Am J Med 84:33–38
Frey HMM, Rosenlund B (1970) Diabetes 19:930–937
Zecca L, Strivulzio S, Pinelli A, Colombo R, Tofanetti O (1985) J Chromatogr 339:203–209
Bakare MT, Mustapha A, Abdu-Aguye I (1994) Chromatographia 31:107–109
Chua HC, Stewart B, Lim BH, Lee HK (1998) J Chromatogr B 712:243–252
Múñez M, Ferguson JE, Machacek D, Jacob G, Oda RP, Lawson GM, Landers JP (1995) Anal Chem 67:3668–3675
Paroni R, Comuzzi B, Arcelloni C, Brocco S, De Kreutzenberg S, Tiengo A, Ciucci A, Beck-Peccoz P, Genovese S (2000) Clin Chem 46:1773–1780
Magni F, Marazzini L, Pereira S, Monti L, Galli Kienle M (2000) Anal Biochem 282:136–141
Kuroda N, Sugihara S, Sugihara Y, Wada M, Kishikawa N, Ohba Y, Nakashima K (2005) J Chromatogr A 1066:119–125
Kishikawa N, Hamachi C, Imamura Y, Ohba Y, Nakashima K, Tagawa Y, Kuroda N (2006) Anal Bioanal Chem 386:719–724
Hammad SF, Mabrouk MM, Habib A, Elfatatry H, Kishikawa N, Nakashima K, Kuroda N (2007) Biomed Chromatogr 21:1030–1035
Adutwum LA, Kishikawa N, Ohyama K, Harada S, Nakashima K, Kuroda N (2010) Anal Bioanal Chem 398:823–829
Kishikawa N, Kubo K, Hammad SF, Mabrouk MM, Habib A, Elfatatry H, Ohyama K, Nakashima K, Kuroda N (2009) J Chromatogr A 1216:6873–6876
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kishikawa, N., Hammad, S.F., Ohyama, K. et al. HPLC Determination of Chlorpropamide in Human Serum by Fluorogenic Derivatization Based on the Suzuki Coupling Reaction with Phenylboronic Acid. Chromatographia 76, 703–706 (2013). https://doi.org/10.1007/s10337-013-2451-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-013-2451-5