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Evaluation of Free Radical-Scavenging Activity of Sea Urchin Pigments Using HPTLC with Post-Chromatographic Derivatization

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Abstract

Chromatographic separation, analysis of in situ radical-scavenging activity, and quantitative analysis of green sea urchin shell pigments were carried out by online HPTLC-DAD and HPTLC-DPPH methods. Methanol:chloroform:acetic acid:water (11:50:5:2, v/v) was selected as the best mobile phase on silica gel plates for the separation of the pigments. Two pigment spots, S1 and S2, were observed on the TLC plates, while echinochrome A was not detected in the pigment extract of green sea urchin shells. Based on the estimated ID50 values, the antiradical activity was S2 (0.043 μg) > S1 (0.058 μg) > echinochrome A (0.134 μg).

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References

  1. Amarowicz R, Synowiecki J, Shahidi S (2012) Food Chem 133:822–826

    Article  CAS  Google Scholar 

  2. Hatate H, Murata H, Hama Y, Tanaka R, Suzuki N (2002) Fish Sci 68(Suppl 2):1641–1642

    Google Scholar 

  3. Lebedev AV, Ivanova MV, Levitsky DO (2008) Hemoglobin 32:165–179

    Article  CAS  Google Scholar 

  4. Kuwahara R, Hatate H, Yuki T, Hisashi MH, Tanaka R, Hama Y (2009) LWT-Food Sci Technol 42:1296–1300

    Article  CAS  Google Scholar 

  5. Mishchenko NP, Fedoreev SA, Bagirova VL (2003) Pharm Chem J 37:48–52

    Article  CAS  Google Scholar 

  6. Anufriev VPh, Novikov VL, Maximov OB, Elyakov GB, Levitsky DO, Lebedev AV, Sadretdinov SM, Shvilkin AV, Afonskaya NI, Ruda MY, Cherpachenko NM (1998) Bioorg Med Chem Lett 8:587–592

    Article  CAS  Google Scholar 

  7. Cieśla Ł, Kryszeń J, Stochmal A, Oleszek W, Waksmundzka-Hajnos M (2012) J Planar Chromatogr 25:225–231

    Article  Google Scholar 

  8. Cieśla Ł, Staszek D, Hajnos M, Kowalska T, Waksmundzka-Hajnos M (2011) Phytochem Anal 22:59–65

    Article  Google Scholar 

  9. Pozharitskaya ON, Ivanova SA, Shikov AN, Makarov VG (2008) Phytochem Anal 19:236–243

    Article  CAS  Google Scholar 

  10. Pozharitskaya ON, Ivanova SA, Shikov AN, Makarov VG (2007) J Sep Sci 30:1250–1254

    Article  CAS  Google Scholar 

  11. Pozharitskaya ON, Ivanova SA, Shikov AN, Makarov VG, Galambosi B (2007) J Sep Sci 30:2447–2451

    Article  CAS  Google Scholar 

  12. Amarowicz R, Synowiecki J, Shahidi F (1994) Food Chem 51:227–229

    Article  CAS  Google Scholar 

  13. Moore RE, Singh H, Scheuer PJ (1966) J Org Chem 31:3645–3650

    Article  CAS  Google Scholar 

  14. Zhou Da-Yong, Qin Lei, Zhu Bei-Wei, Wang Xiao-Dong, Tan Hui, Yang Jing-Feng, Li Dong-Mei, Dong Xiu-Ping, Hai-Tao Wu, Sun Li-Ming, Li Xiu-Ling, Murata Y (2011) Food Chem 129:1591–1597

    Article  CAS  Google Scholar 

  15. Shankarlal S, Prabu K, Natarajan E (2011) Am-Euras J Sci Res 6:178–181

    Google Scholar 

  16. Kuwahara R, Hatate H, Chikami A, Murata H, Kijidani Y (2010) LWT-Food Sci Technol 43:1185–1190

    Article  CAS  Google Scholar 

  17. Shikov AN, Ossipov VI, Martiskainen O, Pozharitskaya ON, Ivanova SA, Makarov VG (2011) J Chromatogr A 1218:9111–9114

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Saint Petersburg Institute of Pharmacy, 47/5, Piskarevskiy pr, 195067, St. Petersburg, Russia

    Olga N. Pozharitskaya, Svetlana A. Ivanova, Alexander N. Shikov & Valery G. Makarov

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  1. Olga N. Pozharitskaya
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  2. Svetlana A. Ivanova
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  3. Alexander N. Shikov
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  4. Valery G. Makarov
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Correspondence to Alexander N. Shikov.

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Published in the topical collection Miniaturized and New Featured Planar Chromatography and Related Techniques with guest editor Paweł K. Zarzycki.

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Pozharitskaya, O.N., Ivanova, S.A., Shikov, A.N. et al. Evaluation of Free Radical-Scavenging Activity of Sea Urchin Pigments Using HPTLC with Post-Chromatographic Derivatization. Chromatographia 76, 1353–1358 (2013). https://doi.org/10.1007/s10337-013-2427-5

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  • DOI: https://doi.org/10.1007/s10337-013-2427-5

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