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Metabonomics Study of TCM Formula: Qutan Huayu Tongmai Granule as an Effective Treatment for Atherosclerosis in Mini-Pigs

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Abstract

To investigate the effects of QuTan HuaYu TongMai granule (QHTG) on the perturbed metabolism of atherosclerosis mini-pig model, a serum metabonomics method based on Liquid chromatography/mass spectrometry was developed. Principal component analysis and partial least squares-discriminate analysis models were established for the metabonomics analysis. The effect of high dose QHTG group is equivalent with those of positive drugs group in PCA score plots. Some significantly changed metabolites, including lyso-PCs, metabolites of vitamin D3 and fatty acids were found to be reasonable in explaining the anti-atherosclerosis mechanism of QHTG. Metabonomic approach is helpful to further understand the pathophysiology of atherosclerosis in mini-pigs and the therapeutic mechanism of QHTG. Our work also indicated that the LC–MS based metabonomics method is a potential tool for performing intervention and mechanism research of traditional Chinese medicine (TCM).

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Acknowledgments

The work was supported financially by National key project of Scientific and Technical Supporting programs of China (No. 2009ZX09502-017), National Natural Science Foundation of China (No. 30830118 and No. 81102795), International technology cooperative program of the People’s Republic of China (No. 2011DFA31440), 49th China Postdoctoral Science Foundation funded project.

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Authors and Affiliations

  1. Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 100091, Beijing, People’s Republic of China

    Sun Ming-Qian, Liu Jian-Xun, Lin Cheng-Ren, Li Lei, Ren Jian-Xun, Miao Lan, Cao Jin & Lin Li

  2. Shenway Pharmaceutical Co. Ltd., 051430, Shijiazhuang, People’s Republic of China

    Sun Ming-Qian

  3. Center of Food and Cosmetics Analysis, National Institute of Food and Drug Control, 100050, Beijing, People’s Republic of China

    Cao Jin

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  1. Sun Ming-Qian
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  2. Liu Jian-Xun
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  3. Lin Cheng-Ren
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  4. Li Lei
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  5. Ren Jian-Xun
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  6. Miao Lan
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  7. Cao Jin
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  8. Lin Li
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Correspondence to Liu Jian-Xun.

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Ming-Qian, S., Jian-Xun, L., Cheng-Ren, L. et al. Metabonomics Study of TCM Formula: Qutan Huayu Tongmai Granule as an Effective Treatment for Atherosclerosis in Mini-Pigs. Chromatographia 75, 1279–1286 (2012). https://doi.org/10.1007/s10337-012-2316-3

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  • DOI: https://doi.org/10.1007/s10337-012-2316-3

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