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Rapid Simultaneous Determination of Four Alkaloids in Lotus Plumule by CZE with Ephedrine Hydrochloride as an Internal Standard

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Abstract

CE offers the possibility of fast, cheap and reproducible separations for active compounds of Chinese herbs. Lotusine (1), liensinine (2), isoliensinine (3) and neferine (4) were the major bioactive alkaloids in lotus plumule, which was used as an important Chinese herb. In this paper, simultaneous separation of 1–4 in lotus plumule by nonaqueous CZE has been achieved within 11 min by use of buffer consisting of 80 mM sodium acetate and 40 mM ammonium acetate methanol solution of pH 5.4. Analysis of the four alkaloids in ten plumule samples of Nelumbo nucifera was conducted. Limits of detection (LOD) of 1–4 by UV absorbance at 203 nm were achieved in the range of 1.5–2.8 μg mL−1. Reproducibility (percent RSD) of the analysis by CZE were 2.33, 2.31, 0.78 and 2.58 %, respectively (n = 5).

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Acknowledgments

The authors would like to thank David Wedge of Natural Products Utilization Research Unit and National Center for Natural Products Research University, MS, USA, for editorial help on the manuscript. This research was supported by Jiangsu Provincial Natural Science Fund (BK2006237), Qing Lan Project (2008′) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Project No. ysxk–2010).

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Authors and Affiliations

  1. National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing, 210046, China

    Guangming Yang, Weidong Li & Yang Pan

  2. Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing and Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210029, China

    Guangming Yang, Weidong Li & Yang Pan

  3. Department of Bio-Pharmacy and Laboratory of Medical Fungi and Phyto-Biotech, Nanjing University of Chinese Medicine, Nanjing, 210029, China

    Yang Pan & Xia Tu

Authors
  1. Guangming Yang
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  2. Weidong Li
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  3. Yang Pan
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  4. Xia Tu
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Correspondence to Yang Pan.

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Yang, G., Li, W., Pan, Y. et al. Rapid Simultaneous Determination of Four Alkaloids in Lotus Plumule by CZE with Ephedrine Hydrochloride as an Internal Standard. Chromatographia 75, 1295–1300 (2012). https://doi.org/10.1007/s10337-012-2312-7

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  • DOI: https://doi.org/10.1007/s10337-012-2312-7

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