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Kinetic Study on the Isomerization of Perindopril by HPLC

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Abstract

The isomerization of perindopril has been investigated using dynamics chromatography and an unified equation introduced by Trapp that was based on stochastic and theoretical plate models to determine the energies. The isomerization rate constants and Gibbs activation energies of isomerization are directly calculated from chromatographic peak parameters, i.e., retention times of the inter-converting species, peak width at half height, and relative plateau height. From the rate constant \( k_{1}^{ue} (T) \), measured at variable temperatures, the kinetic eyring activation parameters ΔG #, ΔH # and ΔS # of isomerization of perindopril were obtained. By variation of the flow rate of the mobile phase, the expected independence of the isomerization barrier from the chromatographic time scale was demonstrated for the first time. The relationships between peak shape and chromatographic conditions, such as flow rate, temperature, pH, organic modifier, and β-cyclodextrin, such as an additive, were investigated. In addition, an NMR investigation on perindopril was described.

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Authors and Affiliations

  1. Laboratoire de Chimie analytique et Electrochimie, Faculté des Sciences de Tunis, 1060, Tunis, Tunisia

    N. Ben Hamida

  2. ACTAPharma, Z. I. oued ezarga, 9013, Béja, Tunisia

    H. Trabelsi

  3. Laboratoire de chimie organique Structurale: Synthèse et Etude Physico-Chimique (Equipe de Chimie de Coordination), Faculté des Sciences de Tunis, 1060, Tunis, Tunisia

    M. T. Ben Dhia

  4. Laboratoire de chimie analytique appliquée, University of Carthage, Faculty of Sciences, Bizerte, 7021, Bizerte, Jarzouna, Tunisia

    S. Bouabdallah

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  1. S. Bouabdallah
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  2. H. Trabelsi
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  3. M. T. Ben Dhia
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  4. N. Ben Hamida
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Correspondence to S. Bouabdallah.

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Bouabdallah, S., Trabelsi, H., Dhia, M.T.B. et al. Kinetic Study on the Isomerization of Perindopril by HPLC. Chromatographia 75, 1247–1255 (2012). https://doi.org/10.1007/s10337-012-2311-8

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  • DOI: https://doi.org/10.1007/s10337-012-2311-8

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