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Identification of the Fatty Acyl Residues Composition and Molecular Species of Phosphatidylcholines in Soy Lecithin Powder by UPLC–ESI-MS/MS

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Abstract

The fatty acyl residues composition and molecular species of phosphatidylcholines (PCs) in soy lecithin powder were studied and characterized using ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC–ESI-MS/MS) via a two-step procedure. ESI-MS/MS by precursor ion scan (PIS) m/z 184.1 in positive ion mode first detected isobaric PCs, and further identified positional isomers and fatty acyl residue composition in negative ion mode. The results showed that the fatty acyl residue composition was in strong agreement with that detected by the classical analysis method. Likewise, ten peaks of isobaric PCs were obtained by ESI-MS/MS analysis in positive ion mode, and 20 positional isomers of PCs were characterized in negative ion mode. Thus, this study presents a simple and powerful way to analyze fatty acyl residue composition and molecular species of PCs without destroying chemical structure.

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Acknowledgments

The work is supported by Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period of China (contract no: 2011BAD02B04), the National Natural Science Foundation of China (NSFC, 31171703) and Program for New Century Excellent Talents in University (NECT). The authors also thank the Testing and Analysis Center of Jiangnan University.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, People’s Republic of China

    Wenming Cao, Kangyi Zhang, Guanjun Tao, Xingguo Wang & Yuanfa Liu

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  1. Wenming Cao
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  2. Kangyi Zhang
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  3. Guanjun Tao
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  4. Xingguo Wang
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  5. Yuanfa Liu
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Corresponding author

Correspondence to Yuanfa Liu.

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W. Cao and K. Zhang contributed equally to this work.

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Cao, W., Zhang, K., Tao, G. et al. Identification of the Fatty Acyl Residues Composition and Molecular Species of Phosphatidylcholines in Soy Lecithin Powder by UPLC–ESI-MS/MS. Chromatographia 75, 1271–1278 (2012). https://doi.org/10.1007/s10337-012-2309-2

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