Abstract
An alternative methodology for simultaneous determination of isoniazid, isonicotinic acid, 4-cyanopyridine, ethyl isonicotinate and isonicotinamide by capillary zone electrophoresis, within an analysis time of 11 min, is proposed. An electrolyte composed by 50 mmol L−1 of acetic acid/sodium acetate buffer and 12.5 mmol L−1 of Cu2+, an analyte dilution in 1 mmol L−1 of Brij 35 and 12.5 mmol L−1 of Cu2+ and +20 kV voltage were optimized. After evaluating some figures of merit, such as linearity, precision, recovery and quantification limit, the method was successfully applied to the isoniazid analysis in tablets. The contents found were 99.3 mg of isoniazid and 0.3 mg of isonicotinic acid, corresponding to 0.3 % of impurity regarding the content of the active ingredient in the pharmaceutical formulation.
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Acknowledgments
The authors wish to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-476386/2007-1 and 300593/2008-2), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG-CEX-APQ 1906-502/07, CEX APQ 01837/08, CEX-PPM 00326/09) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for fellowships and financial support. Special thanks to Nurse Mércia Guadalupe Ramos from the Departamento de Vigilância Epidemiológica from Juiz de Fora for purchasing ISO samples.
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Faria, A.F., Vasconcelos, J.P., Gonçalves, R.S.B. et al. Simultaneous Analysis of Isoniazid and Its Impurities by CZE. Chromatographia 75, 1335–1339 (2012). https://doi.org/10.1007/s10337-012-2308-3
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DOI: https://doi.org/10.1007/s10337-012-2308-3