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GC–MS Based Serum Metabolomic Analysis of Isoflurane-Induced Postoperative Cognitive Dysfunctional Rats: Biomarker Screening and Insight into Possible Pathogenesis

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Abstract

Postoperative cognitive dysfunction (POCD) is a subtle cognitive dysfunction, especially memory impairment for weeks or months after surgery. The underlying pathophysiological mechanism of POCD is still unclear. The aim of this study was to exploratively investigate the potential mechanism of POCD by identifying the differences among metabolic profiles of control rats, POCD and no-POCD rats after isoflurane anesthesia based on GC–MS, and subsequently discovering POCD biomarkers. In this paper, a feature-variable selection method, subwindow permutation analysis (SPA), was employed to seek the key metabolites distinguishing POCD from control group, POCD from no-POCD group. Fortunately, two key metabolites, hexadecanoic acid and myo-Inositol, were both screened out for discriminating POCD and control, POCD and no-POCD rats. It suggested that they may reveal the disturbances between POCD and control, POCD and no-POCD rats, which may be the potential biomarkers of POCD. Furthermore, related possible pathogenesis was taken into account on the basis of the relevant literatures and pathway databases. It suggested that POCD was probably related to disturbed hexadecanoic acid metabolism and myo-Inositol metabolism. All the results demonstrated that the proposed metabolic profiling approach and SPA method may be effective for exploring metabolic perturbations and possible biomarkers for POCD.

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Acknowledgments

This work is financially supported by the National Nature Foundation Committee of P.R. China (Grants No. 20875104, 21105129 and 21075138), the international cooperation project on traditional Chinese medicines of ministry of science and technology of China (Grant No. 2007DFA40680), Special Foundation of China Postdoctoral Science (No. 200902481) and the Fundamental Research Funds for the Central Universities (2010QZZD010 and 2011QNZT053). The studies have been approved by the university’s review board. We are grateful to all employees of this institute for their encouragement and support of this research.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, China

    Wan Zhang, Liangxiao Zhang, Hongdong Li, Yizeng Liang, Nannan Liang, Wei Fan, Dongsheng Cao, Lunzhao Yi & Jidong Xia

  2. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China

    Liangxiao Zhang

  3. Department of Anaesthesia, The Third Xiangya Hospital, Central South University, 410013, Changsha, China

    Rong Hu

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  1. Wan Zhang
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  2. Liangxiao Zhang
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  3. Hongdong Li
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  4. Yizeng Liang
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  5. Rong Hu
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  6. Nannan Liang
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  7. Wei Fan
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  8. Dongsheng Cao
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  9. Lunzhao Yi
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Correspondence to Yizeng Liang or Lunzhao Yi.

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Zhang, W., Zhang, L., Li, H. et al. GC–MS Based Serum Metabolomic Analysis of Isoflurane-Induced Postoperative Cognitive Dysfunctional Rats: Biomarker Screening and Insight into Possible Pathogenesis. Chromatographia 75, 799–808 (2012). https://doi.org/10.1007/s10337-012-2246-0

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  • DOI: https://doi.org/10.1007/s10337-012-2246-0

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