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HPLC-ELSD Determination of Kryptofix 2.2.2 in the Preparations of 2-Deoxy-2-[18F]fluoro-d-glucose and other Radiopharmaceuticals

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Abstract

A new and simple high-performance liquid chromatography with evaporative light scattering detector method for the determination of Kryptofix 2.2.2 (K-222) in the radiopharmaceuticals of 2-deoxy-2-[18F]fluoro-d-glucose ([18F]FDG) and 3′-deoxy-3′-[18F]fluorothymidine ([18F]FLT) was developed. A C18 column was used and the mobile phase was 10 % (v/v) methanol and 90 % (v/v) water (0.1 % trifluoroacetic acid, v/v) at a flow rate of 0.2 mL min−1. The drift tube temperature was 40 °C. The pressure of nebulizing gas (N2) was 3.0 bar. The gain was 10. Good separation of K-222 from main related substances could be achieved. Excellent linearity (r 2 = 0.9995) was obtained over the range of 5–100 μg mL−1. The precision ranged from 0.68 to 5.16 % (RSD) and the accuracy ranged from −3.05 to 2.62 % (RE). The limit of detection was 2 μg mL−1. This method offers simple, rapid and quantitative detection of K-222, thus making it acceptable for routine determination.

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Acknowledgments

This research was supported by grants from Guangzhou Science and Technology Bureau, People’s Republic of China (No. 2009Z1-E371).

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Authors and Affiliations

  1. Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, 510010, Guangzhou, People’s Republic of China

    Weijie Deng, Xiangnian Zhang, Zhiping Sun, Liping Han & Shujin Zhao

  2. Department of Nuclear Medicine, Guangzhou General Hospital of Guangzhou Military Command, 510010, Guangzhou, People’s Republic of China

    Jilin Yin & Zheng Zhou

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  1. Weijie Deng
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  2. Xiangnian Zhang
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  3. Zhiping Sun
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  4. Jilin Yin
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  5. Zheng Zhou
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  6. Liping Han
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  7. Shujin Zhao
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Correspondence to Shujin Zhao.

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Deng, W., Zhang, X., Sun, Z. et al. HPLC-ELSD Determination of Kryptofix 2.2.2 in the Preparations of 2-Deoxy-2-[18F]fluoro-d-glucose and other Radiopharmaceuticals. Chromatographia 75, 629–633 (2012). https://doi.org/10.1007/s10337-012-2242-4

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  • DOI: https://doi.org/10.1007/s10337-012-2242-4

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