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Preparation of Titania Monolith Column and Application in Determination of Benzoic Acid by HILIC

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Abstract

The titania monolith column has been synthesized through a template-free sol–gel route, and a simple and reliable method for the determination of benzoic acid by hydrophilic interaction liquid chromatography using the prepared titania monolith has been developed. The influences of acetonitrile, acetate buffer and buffer pH on the retention of benzoic acid were investigated. Benzoic acid in carbonated drinks and fruit beverages samples were determined within 5 min and quantitative analysis was carried out by external standard method with a correlation coefficient (R 2) of 0.9984. The relative standard deviation was 0.91 % and the recovery ranged from 92.5 to 101.3 %. The proposed method is suitable for the analysis of benzoic acid in beverage samples.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 20875069), the Science Foundation for Young Teachers of Tianjin University of Commerce (No. 090107), and Natural Science Foundation of Tianjin.

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Authors and Affiliations

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300134, People’s Republic of China

    Jia Wei, Zi-Tao Jiang, Rong Li & Jin Tan

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  1. Jia Wei
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  2. Zi-Tao Jiang
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  3. Rong Li
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  4. Jin Tan
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Correspondence to Zi-Tao Jiang.

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Wei, J., Jiang, ZT., Li, R. et al. Preparation of Titania Monolith Column and Application in Determination of Benzoic Acid by HILIC. Chromatographia 75, 563–569 (2012). https://doi.org/10.1007/s10337-012-2230-8

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  • DOI: https://doi.org/10.1007/s10337-012-2230-8

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