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Development of an HPLC Method for Determination of Related Impurities in Prilocaine Substance

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Abstract

Development of a reversed phase high performance liquid chromatographic method for determination of six related impurities in prilocaine substance is reported. The test of related impurities in European Pharmacopoeia (Ph. Eur.) cannot meet the demands with the chromatographic parameters given, therefore different types of chromatographic systems and eight columns have been evaluated in the present study. A new method with a Hypercarb column was developed and validated. This method fulfils the demands in the Ph. Eur., and the validation shows that the method is selective, reproducible, linear, accurate and robust with sufficient limits of detection (0.001–0.004% of 2.5 mg prilocaine mL−1) and quantification (0.002–0.009% of 2.5 mg prilocaine mL−1).

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References

  1. McCalley DV (1999) J Chromatogr A 844:23–38

    Article  CAS  Google Scholar 

  2. Vervoort RJM, Reyter E, Debets AJJ, Claessens HA, Cramers CA, deJong GJ (2001) J Chromatogr A 931:67–79

    Google Scholar 

  3. Cruz E, Euerby MR, Johnson CM, Hackett CA (1997) Chromatographia 44:151–161

    Article  CAS  Google Scholar 

  4. Euerby M, Petersson P (2000) LCGC 18(1):665–677

    Google Scholar 

  5. Euerby MR, Petersson P (2003) J Chromatogr A 994:13–36

    Article  CAS  Google Scholar 

  6. Euerby MR, Petersson P (2005) J Chromatogr A 1088:1–15

    Article  CAS  Google Scholar 

  7. Euerby MR, Petersson P, Campbell W, Roe W (2007) J Chromatogr A 1154:138–151

    Article  CAS  Google Scholar 

  8. Markopoulou C, Tweelie T, Watson D, Skellern G, Reda H, Petersson P, Bradstock H, Euerby M (2009) Chromatographia 70:705–715

    Article  CAS  Google Scholar 

  9. Wan QH, Shaw PN, Davies MC, Barrett DA (1995) J Chromatogr A 697:219–227

    Article  CAS  Google Scholar 

  10. Ayrton J, Evans MB, Harris AJ, Plumb RS (1995) J Chromatogr B 667:173–178

    Article  CAS  Google Scholar 

  11. Huynh N, Karlsson A, Pettersson C (1995) J Chromatogr A 705:275–287

    Article  CAS  Google Scholar 

  12. Karlsson A, Charron C (1996) J Chromatogr A 732:245–253

    Article  CAS  Google Scholar 

  13. Karlsson A, Berglin M, Charron C (1998) J Chromatogr A 797:75–82

    Article  CAS  Google Scholar 

  14. Gyllenhaal O, Karlsson A (2010) Chromatographia 71:7–13

    Article  CAS  Google Scholar 

  15. Xia Y, Jemal M, Zheng N, Shen X (2006) Rapid Commun Mass Spetrom 20:1831–1837

    Article  CAS  Google Scholar 

  16. Beynon RJ, Easterby JS (1996) Buffer solutions, Oxford Press, pp 65–66

  17. Kirkland JJ, Adams JB, van Straten MA, Claessens HA (1998) Anal Chem 70:4344

    Article  CAS  Google Scholar 

  18. Gilbert MT, Knox JH, Kaur B (1982) Chromatographia 16:138–146. doi:10.1007/BF02258884

    Article  CAS  Google Scholar 

  19. Jacquet R, Pennanec R, Elfakir C, Lafosse M (2004) J Sep Sci 27:1221–1228. doi:10.1002/jssc.200401758

    Google Scholar 

  20. Knox JH, Ross P (1997) Carbon-based packing materials for liquid chromatography. Structure, performance and retention mechanisms. In: Brown PR, Grushka E (eds) Advances in chromatography, vol 37. Marcel Dekker, New York, pp 73–119

    Google Scholar 

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Authors and Affiliations

  1. Analytical Development, AstraZeneca, 15185, Södertälje, Sweden

    Janina Sroka-Markovic, Linda Johansson & Magnus B. O. Andersson

  2. Department of Analytical Chemistry, Stockholm University, 10691, Stockholm, Sweden

    Ingrid Granelli

Authors
  1. Janina Sroka-Markovic
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  2. Linda Johansson
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  3. Magnus B. O. Andersson
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  4. Ingrid Granelli
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Correspondence to Ingrid Granelli.

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Sroka-Markovic, J., Johansson, L., Andersson, M.B.O. et al. Development of an HPLC Method for Determination of Related Impurities in Prilocaine Substance. Chromatographia 75, 329–336 (2012). https://doi.org/10.1007/s10337-012-2212-x

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  • DOI: https://doi.org/10.1007/s10337-012-2212-x

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