Skip to main content
SpringerLink
Log in

UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Determination of the levels of 1-octacosanol is important in food stuff for the study of its pharmacological activities and health benefits. In this study, a novel, simple and fast internal standard method for the non-derivatization ultra-performance liquid chromatographic determination of 1-octacosanol in raw materials and health products was developed and validated based on evaporative light scattering detection. The linearity (r 2 > 0.998), recovery (99.1–100.2%, RSD <2.7%), intra- and inter-day precision (RSD <3.8%), limit of detection (1.0 mg/L), limit of quantification (2.2 mg/L) of the 1-octacosanol were determined. The method was successfully applied to nine real 1-octacosanol products. The results of analyses had close agreement with the labeled claims of 1-octacosanol content in these products. Compared with the classical gas chromatography method, the developed method was simpler, faster and more environmentally friendly due to avoiding any derivatization step. This protocol represents a rapid and feasible method for quality control of 1-octacosanol products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

References

  1. Arruzazabala ML, Carbajal D, Mas R, Molina V, Valdes S, Laguna A (1994) Biol Res 27:205–208

    CAS  Google Scholar 

  2. Kato S, Karino K, Hasegawa J, Nagasaki A, Eguchi M, Ichinose T (1995) Br J Nutr 73:433–441

    Article  CAS  Google Scholar 

  3. Chen F, Cai T, Zhao G, Liao X, Guo L, Hu X (2005) J Food Eng 70:47–53

    Article  Google Scholar 

  4. Gonzalez L, Magraner J, Acosta PC, Perez N (1996) J Chromatogr B 682:359–363

    Article  Google Scholar 

  5. Delange DM, Bravo LG (2001) J Chromatogr B 762:43–49

    Article  Google Scholar 

  6. Haim D, Berrios M, Valenzuela A, Videla LA (2009) J Chromatogr B 877:4154–4158

    Article  CAS  Google Scholar 

  7. Park MK, Park JH, Han SB, Shen YG, Park IH (1996) J Chromatogr A 736:77–81

    Article  CAS  Google Scholar 

  8. Mourney TH, Oppenheimer LE (1984) Anal Chem 56:2427–2434

    Article  Google Scholar 

  9. Kong W, Jin C, Liu W, Xiao X, Zhao Y, Li Z, Zhang P, Li X (2010) Food Chem 120:1193–1200

    Article  CAS  Google Scholar 

  10. Kong W, Jin C, Xiao X, Zhao Y, Liu W, Li Z, Zhang P (2010) J Sep Sci 33:1518–1527

    Article  CAS  Google Scholar 

  11. Villiers A, Gorecki T, Lynen F, Szucs R, Sandra P (2007) J Chromatogr A 1161:183–191

    Article  Google Scholar 

  12. Megoulas NC, Koupparis MA (2004) J Pharm Biomed Anal 36:73–79

    Article  CAS  Google Scholar 

  13. Nederkassel AM, Vijverman V, Massart DL, Vander Heyden Y (2005) J Chromatogr A 1085:230–239

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the 948 project (No. 2010-G1) from the Ministry of Agriculture of China and a special fund (CARS-45) from Apicultural Industry Technology System Construction of Chinese Modern Agriculture.

Author information

Authors and Affiliations

  1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100093, China

    Xiaofeng Xue, Lanzhen Chen, Jinhui Zhou, Liming Wu, Yi Li, Fang Chen, Jinzhen Zhang & Jing Zhao

  2. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100081, China

    Xiaofeng Xue

  3. Apicultural Branch Center, Research and Development Center of National Agro-food Processing Technology, Beijing, 102202, China

    Xiaofeng Xue, Lanzhen Chen, Jinhui Zhou, Liming Wu, Yi Li, Fang Chen, Jinzhen Zhang & Jing Zhao

Authors
  1. Xiaofeng Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lanzhen Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jinhui Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liming Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jinzhen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jing Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jing Zhao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xue, X., Chen, L., Zhou, J. et al. UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products. Chromatographia 75, 165–168 (2012). https://doi.org/10.1007/s10337-011-2166-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-011-2166-4

Keywords

Search

Navigation