Skip to main content
SpringerLink
Log in

Nafion®-Separated Electrode Solutions in Isoelectric Focusing

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

In the present paper, we present our results from studies where the electrode solutions were separated from the carrier with the polytetrafluoroethylene-based membranes (Nafion®). We achieved a 40-fold decrease of the average ionic strength in the gel and a twofold lowering of the current already during the first 30 min from the start of IEF, as compared to the routinely employed method. The change of these parameters made it possible to carry out the electrophoresis under conditions considerably closer to steady state, and to achieve a sharp protein separation and shortening of the duration of the process. The comparative analysis of the electrophoretic parameters in question proved that the basis for this newly developed improvement of the method is the selective restriction of the processes of migration and diffusion in the whole electrophoretic system, due to the specific properties of the semi-permeable membrane Nafion®.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Baumann GA, Chrambach A (1975) In: Righetti P (ed) Progress in Isoelectric Focusing and Isotachophoresis, Elsevier Exepta Medica, Holland

  2. Nguyen NY, Chrambach A (1980) Electrophoresis 1:14–22

    Article  CAS  Google Scholar 

  3. Palusinski OA, Bier M, Saville DA (1981) Biophys Chem 14:389–397

    Article  CAS  Google Scholar 

  4. Bier M, Mosher RA, Palusinski OA (1981) J Chromatogr 211:313–335

    Article  CAS  Google Scholar 

  5. Bier M, Palusinski OA, Mosher RA, Saville DA (1983) Science 219:1281–1287

    Article  CAS  Google Scholar 

  6. Mosher RA, Dewey D, Thormann W, Saville DA, Bier M (1989) Anal Chem 61:362–366

    Article  CAS  Google Scholar 

  7. Sova O (1985) J Chromatogr 320:15–22

    Article  CAS  Google Scholar 

  8. Pospichal J, Glovinova E (2001) J Chromatogr A 918:195–203

    Article  CAS  Google Scholar 

  9. Shang TQ, Ginter JM, Johnston MV, Larsen BS, McEwen CN (2003) Electrophoresis 24:2359–2368

    Article  CAS  Google Scholar 

  10. Stoyanov AV, Champak D, Fredrickson CK, Hugh Fan Z (2005) Electrophoresis 26:473–479

    Article  CAS  Google Scholar 

  11. Stoyanov AV, Righetti PG (1999) Electrophoresis 20:718–722

    Article  CAS  Google Scholar 

  12. Bier M, Mosher RA, Palusinski OA (1981) J Chromatogr 211:313–335

    Article  CAS  Google Scholar 

  13. Bier M, Palusinski OA, Mosher RA, Saville DA (1983) Science 219:1281–1287

    Article  CAS  Google Scholar 

  14. Mosher RA, Dewey D, Thormann W, Saville DA, Bier M (1989) Anal Chem 61:362–366

    Article  CAS  Google Scholar 

  15. Hagedorn R, Fuhr G (1990) Electrophoresis 11:281–289

    Article  CAS  Google Scholar 

  16. Mosher RA, Saville DA, Thormann W (1992) The dynamics of electrophoresis. VCH, Weinheim

    Google Scholar 

  17. Mosher RA, Thormann W, Bier M (1986) J Chromatogr 351:31–38

    Article  CAS  Google Scholar 

  18. Mosher RA, Thormann W, Bier M (1988) J Chromatogr 436:191–204

    Article  CAS  Google Scholar 

  19. Pospichal J, Deml M, Bocek P (1993) J Chromatogr 638:179–186

    Article  CAS  Google Scholar 

  20. Cao Cheng-Xi (1998) J Chromatogr A 813:153–171

    Article  CAS  Google Scholar 

  21. Cao Cheng-Xi (1998) J Chromatogr A 813:173–177

    Article  CAS  Google Scholar 

  22. Cheng-Xi Cao, Shu-Lin Zhou, You-Zhao He, Xiao-Yun Zheng, Wen-Kui Chen, Yi-Tai Qian (2000) J Chromatogr A 891: 337–347

  23. Cheng-Xi Cao, Shu-Lin Zhou, You-Zhao He, Yi-Tai Qian, Li Yang, Qi-Shu Qu, Wu-Er Gan, Lin Dong, Yao-Qian Zhao, Wen-Kui Chen (2001) J Chromatogr A 907: 347-352

  24. Cheng-Xi Cao, Liu-Yin Fan and Wei Zhang (2008) Analyst 133: 1139-1157

    Google Scholar 

  25. Naydenov CL, Kirazov EP, Kirazov LP, Genadiev TT (2006) J Chromatogr A 1121:129–139

    Article  CAS  Google Scholar 

  26. Naydenov CL, Kirazov E, Lozanov V, Kirazov L, Mitev V (2009) Chromatographia 69:887–895

    Article  CAS  Google Scholar 

  27. Naydenov CL, Kirazov E, Lozanov V, Kirazov L, Mitev V (2010) Chromatographia 72:481–487

    Article  CAS  Google Scholar 

  28. Nafion® is a registered trademark of E.I. DuPont de Nemours & Co

  29. Robertson MAF (1994) Ph.D. Thesis. Department of Chemistry, University of Calgary, Calgary

  30. Yeager HJ, Eisenberg A (1982) In: Eisenberg A, Yeager HL (eds) Perfluorinated ionomer membranes. ACS Symposium Serial No.180, American Chemical Society, Washington DC

  31. Mauritz KA, Storey RF, Jones CK (1989) In: Utracki LA, Weiss RA (eds) Multiphase polymer materials: blends, ionomers, and interpenetrating networks. ACS Symposium Serial No. 395, American Chemical Society, Washington DC

  32. Mauritz KA, Hora CJ, Hopfinger AJ (1980) In: Eisenberg A (ed) Ions in polymers. ACS Advances in Chemistry Ser. No. 187, American Chemical Society, Washington DC

  33. Mauritz KA (1988) JMS Rev Macromol Chem Phys C28(1):65–98

    Article  CAS  Google Scholar 

  34. Eisenberg A, King M (1977) In: Ion-containing polymers: physical properties and structure, Academic Press, New York

  35. Sondheimer SJ, Bunce NJ, Fyfe CA (1986) JMS Rev Macromol Chem Phys C26(3):353–413

    Article  Google Scholar 

  36. Righetti PG, Drysdale JW (1974) J Chromatogr 98:271–321

    Article  CAS  Google Scholar 

  37. Erlandsson PG, Robinson ND (2011) Electrophoresis 32(6–7):784–790

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Medical University Sofia, 2 Zdrave Str., 1431, Sofia, Bulgaria

    Cyrill L. Naydenov, Valentin S. Lozanov & Vanio I. Mitev

  2. Institute of Experimental Morphology and Anthropology With Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 25, 1113, Sofia, Bulgaria

    Evgeni P. Kirazov & Ludmil P. Kirazov

  3. Section of Genetics, Department of Pediatrics, University of California Davis, Sacramento, CA 95618, USA

    Simeon A. Boyadjiev

Authors
  1. Cyrill L. Naydenov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Evgeni P. Kirazov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Simeon A. Boyadjiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ludmil P. Kirazov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Valentin S. Lozanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vanio I. Mitev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cyrill L. Naydenov.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Naydenov, C.L., Kirazov, E.P., Boyadjiev, S.A. et al. Nafion®-Separated Electrode Solutions in Isoelectric Focusing. Chromatographia 75, 33–40 (2012). https://doi.org/10.1007/s10337-011-2158-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-011-2158-4

Keywords

Search

Navigation