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LC–MS–MS Determination of Gizzerosine in Poultry Feed

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Abstract

A new selective method for the determination of the bioactive amine gizzerosine in poultry feed is presented based on hydrophilic interaction chromatography coupled to electrospray ionisation and tandem quadrupole mass spectrometry. The protonated molecular ions were selected for fragmentation and scanning of the two major product ions using the mass analyser in the multiple reaction monitoring mode. The analytical methodology included acid hydrolysis of feed sample aliquots and desalting and crude clean-up of hydrolysates using cation-exchange chromatography on a polymeric solid-phase extraction sorbent. Mean overall recoveries (n = 9) were 68–82% in spike-recovery experiments. The limit of detection was 0.25 mg kg−1. The method was applied to the analysis of fish meal and fish meal-containing poultry feed samples. Although gizzerosine could not be detected in any of the samples, it was found in fish meal made from sand eel after heating to 130 °C for 24 h.

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Acknowledgments

This study was financially supported by the Research Council of Norway, Grant NFR 173367/I10. Dr. Hiromasa Kiyota (Tohuku University, Sendai, Japan) is acknowledged for kindly providing synthetic (S)-gizzerosine.

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Authors and Affiliations

  1. Section for Chemistry and Toxicology, Norwegian Veterinary Institute, P.O. Box 750, Sentrum, 0106, Oslo, Norway

    Silvio Uhlig

  2. Section for Pathology, Norwegian Veterinary Institute, P.O. Box 750, Sentrum, 0106, Oslo, Norway

    Magne Kaldhusdal & Anne-Gerd Gjevre

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  1. Silvio Uhlig
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  2. Magne Kaldhusdal
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  3. Anne-Gerd Gjevre
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Correspondence to Silvio Uhlig.

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Uhlig, S., Kaldhusdal, M. & Gjevre, AG. LC–MS–MS Determination of Gizzerosine in Poultry Feed. Chromatographia 74, 133–138 (2011). https://doi.org/10.1007/s10337-011-2054-y

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  • DOI: https://doi.org/10.1007/s10337-011-2054-y

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