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Determination of Imidol in Rat Plasma by UPLC–MS–MS and Its Application in a Pharmacokinetic Study

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Abstract

A rapid, sensitive and accurate ultra-performance liquid chromatography/tandem mass spectrometry method was developed and validated for the quantitative determination of imidol in rat plasma for the first time. The analyte and internal standard were extracted from plasma by liquid–liquid extraction with diethyl ether. The separation was performed on a BEH C18 column (50 mm × 2.1 mm, 1.7 μm). The detection was carried out by electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring. Linear calibration curves were obtained in the concentration range of 2.5–2,500 ng mL−1, with the lower limit of quantification of 2.5 ng mL−1. The intra- and inter-day precision (RSD) values were below 8% and accuracy (RE) was from −7.9 to 6.3%. After strict validation, the method was applied successfully to the pharmacokinetic study of imidol in rats after oral and intravenous administration, respectively.

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Acknowledgment

This work was supported by a grant from the National Key Scientific Project for New Drug Discovery and Development (Grant No. 2009ZX09301-012).

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China

    Zhong-Bo Liu, Qing Li, Wei Li, Xiao-Wei Song, Guang-Ping Rong, Kai-Shun Bi & Xiao-Hui Chen

  2. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China

    Yan-Fang Zhao & Ping Gong

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  1. Zhong-Bo Liu
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  2. Qing Li
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  3. Wei Li
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  4. Xiao-Wei Song
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  5. Guang-Ping Rong
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  6. Yan-Fang Zhao
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  7. Ping Gong
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  8. Kai-Shun Bi
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  9. Xiao-Hui Chen
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Correspondence to Xiao-Hui Chen.

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Liu, ZB., Li, Q., Li, W. et al. Determination of Imidol in Rat Plasma by UPLC–MS–MS and Its Application in a Pharmacokinetic Study. Chromatographia 74, 59–66 (2011). https://doi.org/10.1007/s10337-011-2035-1

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  • DOI: https://doi.org/10.1007/s10337-011-2035-1

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