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MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix

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Abstract

The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26 μg L−1.

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Acknowledgments

This work was supported by the Research Project of the Ministry of Education of the Czech Republic (grant no. MSM6198959216), by the grant from Palacký University in Olomouc, Czech Republic, FZV_2010_001, and from the Operational Program Research and Development for Innovations—European Social Fund (project CZ.1.05/2.1.00/03.0058 of the Ministry of Education, Youth and Sports of the Czech Republic).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc, 77146, Czech Republic

    Joanna Znaleziona & Juraj Ševčík

  2. Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, Olomouc, 77146, Czech Republic

    Vítězslav Maier & Jan Petr

  3. Faculty of Health Sciences, Palacký University in Olomouc, Tr. Svobody 8, Olomouc, 77146, Czech Republic

    Jan Chrastina

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  1. Joanna Znaleziona
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  2. Vítězslav Maier
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  3. Jan Petr
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  4. Jan Chrastina
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  5. Juraj Ševčík
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Correspondence to Vítězslav Maier.

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Znaleziona, J., Maier, V., Petr, J. et al. MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix. Chromatographia 74, 151–155 (2011). https://doi.org/10.1007/s10337-011-2019-1

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  • DOI: https://doi.org/10.1007/s10337-011-2019-1

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