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Evaluation of Generic Gradients, Sample Pooling and MS Detection as Chiral Resolution Screening Strategies on Diverse Chiral Stationary Phases

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Abstract

Increased throughput in medicinal chemistry has substantially increased the production of new chiral chemical entities with diverse structural features, requiring fast chiral screening strategies. In an effort to explore screening methods we have developed and evaluated gradients in polar organic and normal phase modes on three popular chiral stationary phases using a sample set of 19 racemic compounds. When compared, gradients performed on par with the isocratic methods besides proving to be advantageous in terms of speed of analysis and elution capacity. MS detection was successfully applied to the analysis of a pooled sample consisting 19 compounds to speed-up the screening process.

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Acknowledgments

Thanks are due to Drs. Rashmi Barbhaiya and Kasim Mookhtiar for permitting us to carry out research work towards a Ph.D. Authors would like to thank Hikal and IPCA laboratories for the gift samples.

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Authors and Affiliations

  1. Drug Discovery Facility, Advinus Therapeutics, Quantum Towers, Plot-9, Rajiv Gandhi Infotech Park, Phase-I Hinjewadi, Pune, 411 057, India

    Mahesh Kumar Mone

  2. Department of Chemistry, Jawaharlal Nehru Technological University, College of Engineering, Anantapur, 515 002, India

    Mahesh Kumar Mone & K. B. Chandrasekhar

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  1. Mahesh Kumar Mone
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  2. K. B. Chandrasekhar
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Correspondence to Mahesh Kumar Mone.

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Mone, M.K., Chandrasekhar, K.B. Evaluation of Generic Gradients, Sample Pooling and MS Detection as Chiral Resolution Screening Strategies on Diverse Chiral Stationary Phases. Chromatographia 73, 985–992 (2011). https://doi.org/10.1007/s10337-011-1980-z

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  • DOI: https://doi.org/10.1007/s10337-011-1980-z

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