Abstract
An ion exchange-reversed-phase two-dimensional liquid chromatography (2D-LC) system was developed based on a newly designed interface. The interface was constructed by alternately connecting tandem columns to one of the parallel separation columns to increase the separation efficiency. Separation on the tandem column does not affect the switching frequency; the valve switching could be maintained at high frequency. The constructed interface could be used in both ambient and elevated temperature modes. Under high-temperature mode, only the tandem column was heated, which simplified the operation, decreased the backpressure, improved the solvent compatibility, and speeded up the separation. Tryptic digestion of four proteins was used to evaluate the performance of the developed system. The peak capacity of the 2D-LC system reached 650 under ambient conditions and 890 under high temperature conditions. When compared with the traditional parallel column interface constructed in this paper, the new interface produced a 60% higher peak capacity.
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Guiochon G, Marchetti N, Mriziq K, Shalliker RA (2008) J Chromatogr A 1189:109–168. doi:10.1016/j.chroma.2008.01.086
Zhang WB, Zhang LH, Zhang YK (2007) Chin Bull Life Sci 19:281
Shellie RA, Haddad PR (2006) Anal Bioanal Chem 386:405–415. doi:10.1007/s00216-006-0516-0
Erni F, Frei RW (1978) J Chromatogr A 149:561–569. doi:10.1016/S0021-9673(00)81011-0
Bushey MM, Jorgenson JW (1990) Anal Chem 62:161–167. doi:10.1021/ac00201a015
Dugo P, Cacciola F, Kumm T, Dugo G, Mondello L (2008) J Chromatogr A 1184:353–368. doi:10.1016/j.chroma.2007.06.074
Schoenmakers P, Marriott P, Beens J (2003) LC GC Europe 16:335–339
Shalliker RA, Gray MJ (2006) Adv Chromatogr 44:177–236
Tanaka N, Kimura H, Tokuda D, Hosoya K, Ikegami T, Ishizuka N, Minakuchi H, Nakanishi K, Shintani Y, Furuno M, Cabrera K (2004) Anal Chem 76:1273–1281. doi:10.1021/ac034925j
Ikegami T, Hara T, Kimura H, Kobayashi H, Hosoya K, Cabrera K, Tanaka N (2006) J Chromatogr A 1106:112–117. doi:10.1016/j.chroma.2005.10.068
Huidobro AL, Pruim P, Schoenmakers P, Barbas C (2008) J Chromatogr A 1190:182–190. doi:10.1016/j.chroma.2008.02.114
Li DX, Tang T, Wang FY, Li T, Zhang WB (2008) Chem Online (in Chinese) 2:128–131
Stoll DR, Carr PW (2005) J Am Chem Soc 127:5034–5035. doi:10.1021/ja050145b
Stoll DR, Cohen JD, Carr PW (2006) J Chromatogr A 1122:123–137. doi:10.1016/j.chroma.2006.04.058
Dugo P, Cacciola F, Herrero M, Donato P, Mondello L (2008) J Sep Sci 31:3297–3308. doi:10.1002/jssc.200800281
Wu J, Fan LY, Zhang W, Wang QL, Cao CX (2008) Chin J Chromatogr (in Chinese) 5:622–625
Wolters DA, Washburn MP, Yates JR (2001) Anal Chem 73:5683–5690. doi:10.1021/ac010617e
Liu HJ, Berger SJ, Chakraborty AB, Plumb RS, Cohen SA (2002) J Chromatogr B 782:267–289. doi:10.1016/S1570-0232(02)00554-8
Acknowledgments
We are thankful to the NSFC (no. 20675083), 973 Program (no. 2007CB914100), and Key Projects in the National Science and Technology Pillar Program during the 11th Five-Year Plan Period (no. 2006BAF07B03) for financial support.
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Li, D., Zhang, L., Wang, Z. et al. Development of a Parallel-Tandem Column Interface in a Two-Dimensional Liquid Chromatography System. Chromatographia 73, 871–877 (2011). https://doi.org/10.1007/s10337-011-1974-x
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DOI: https://doi.org/10.1007/s10337-011-1974-x