Abstract
A simple, rapid and highly sensitive capillary ion chromatographic method for direct determination of iodide and thiocyanate is reported. Separation was achieved on a laboratory-made capillary column (100 mm × 0.32 mm i.d.) packed with silica gel, followed by modification with 20 mM hexadimethrine bromide. Sodium perchlorate–methanol (95:5, v/v) was used as the eluent and analyte anions were detected at 225 nm. Iodate, bromate, nitrate, iodide and thiocyanate were eluted within 8 min, with relative standard deviations of the retention time, peak area and peak height smaller than 2.4%. Effects of the eluent composition on the retention behavior were also investigated. The limit of detection (S/N = 3) of iodide was 6.5 μg L−1, whereas that of thiocyanate was 16.2 μg L−1. The method was successfully applied to the rapid and direct determination of iodide in powdered milk and thiocyanate in human saliva without any pre-concentration. The modified column could be used for about 1 month (8 h operation per day) without loss of hexadimethrine bromide.
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References
Hassaniem MH, Hussein LA, Robinson EN, Mercer LP (2003) J Nutr Biochem 14:280–287
Sandstöm B, Lyhne N, Pedersen JI, Aro A, Thorsdottir I, Becker W (1996) Scand J Nutr 40:161–165
Tonacchera M, Pinchera A, Dimida A, Ferrarini E, Agretti P, Vitti P, Santini F, Crump K, Gibbs J (2004) Thyroid 14:1012–1019
Groef BD, Decallonne BR, Van der Geyten S, Darras VM, Bouillon R (2006) Eur J Endocrinol 155:17–25
Okafor PN, Okorowkwo CO, Maduagwu EN (2002) Food Chem Toxicol 40:1001–1005
Glatz Z, Nováková S, Štěrbová H (2001) J Chromatogr A 916:273–277
Kuban P, Dasgupta PK (2004) J Sep Sci 27:1441–1457
Takeuchi T (2005) Chromatographia 26:7–10
Ríordáin CO, Gillespie E, Connolly D, Nesterenko PN, Paull B (2007) J Chromatogr A 1142:185–193
Sugrue E, Nesterenko PavelN, PN PaullB (2005) J Chromatogr A 1075:167–175
Rong L, Takeuchi T (2004) J Chromatogr A 1042:131–135
Rong L, Lim LW, Takeuchi T (2006) J Chromatogr A 1128:68–72
Rong L, Lim LW, Takeuchi T (2007) Talanta 72:1625–1629
Kuban P, Dasgupta PK (2004) J Sep Sci 27:1441–1457
Pyo D, Kim H (2001) Microchem J 70:159–163
Suzuki A, Lim LW, Hiroi T, Takeuchi T (2006) Talanta 70:190–193
Zakaria P, Hutchinson JP, Avdalovic N, Liu Y, Haddad PR (2005) Anal Chem 77:417–423
Millot MC, Debranche T, Pantazaki A, Gherghi I, Sébille B, Vidal-Madjar C (2003) Chromatographia 58:365–373
Hagberg J (2003) J Chromatogr A 988:127–133
Jensen AG, Hansen SH (2002) J Pharm Biomed Anal 27:167–176
Bord N, Crétier G, Rocca JL, Bailly C, Souchez JP (2005) J Chromatogr A 1100:223–229
Li HB, Chen F, Xu XR (2001) J Chromatogr A 918:335–339
Sankaran C, Bhavaniamma V, Satrugnan K (1998) Mikrochim Acta 128:75–77
Takeuchi T, Ishii D (1981) J Chromatogr 213:25–32
Safni, Takeuchi T, Miwa T, Hashimoto Y, Moriyama H (1999) J Chromatogr A 850: 65-72
Collins KD, Washabaugh MW (1985) Q Rev Biophys 18:323–422
Chai CW, Liu KN, Mou SF (2001) Chin J Chromatogr 19:94–96
Tsuge K, Kataoka M, Seto Y (2000) J Health Sci 46:343–350
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This work was a project supported by scientific research fund of SiChuan Provincial Education Department, grant No. 09233023.
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Rong, L., Liu, Z., Ma, M. et al. Determination of Inorganic Anions by Capillary IC Using Hexadimethrine Bromide-Modified Silica Stationary Phases. Chromatographia 73, 501–506 (2011). https://doi.org/10.1007/s10337-011-1927-4
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DOI: https://doi.org/10.1007/s10337-011-1927-4